Oxidative damage to lipids and proteins induced by aflatoxin B1 in fish (Labeo rohita)-protective role of Amrita Bindu

被引：28

作者：

Madhusudhanan, N ^{[1
]}

KavithaLakshmi, SN ^{[1
]}

Shanmugasundaram, KR ^{[1
]}

Shanmugasundaram, ERB ^{[1
]}

机构：

[1] Univ Madras, Dept Med Biochem, Dr ALM Post Grad Basic Med Sci, Madras 600113, Tamil Nadu, India

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2004年 / 17卷 / 02期

关键词：

conjugated dienes; lipid peroxidation; protein carbonyls; aflatoxin B-1; Amrita bindu; Labeo rohita;

D O I：

10.1016/j.etap.2004.03.002

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In the present study, fish (Labeo rohita) were treated with a single intraperitoneal administration of aflatoxin B-1 (AFB(1))(100 mug/100g BW). The resultant oxidative damage to lipids (measured as conjugated diene and lipid peroxidation (LPO)) and proteins (protein carbonyl) in liver, kidney and brain at the end of 3rd and 6th day was assessed. Our results showed that AFB(1) induced a significant increase in conjugated diene formation and LPO notonly in liver but also in kidney and brain. A parallel increase in protein carbonyl level was observed in these tissues. When 1: 1 mixture of 20% solution of Amrita Bindu (a salt-spice-herbal mixture based on Indian system of medicine) was co-administered along with 100 mug AFB(1), the AFB(1) induced increase in conjugated diene, LPO and protein carbonylation were minimised to a greater extent. These results led to conclusion that (i) AFB(1) not only induces oxidative damage to the primary target organ-liverin L. rohita, but also in kidney and brain, (ii) co-administration of Amrita Bindu confers protection to lipids and protein against the AFB(1) induced oxidative damage in all the three tissues. (C) 2004 Published by Elsevier B.V.

引用

页码：73 / 77

页数：5