Unmodified silver nanoparticles for rapid analysis of the organophosphorus pesticide, dipterex, often found in different waters

A novel, uncomplicated and rapid method of analysis for organophosphorus (OP) pesticides was researched and developed using the important, common OP, dipterex, as a typical example. The basis of the method involved the citrate-capped silver nanoparticles (citrate-capped AgNPs) and Acetylthiocholine (ATCh). The latter compound can be catalyzed by Acetylcholine sterase (AChE) to form thiocholine (TCh), which induces the aggregation of AgNPs. Correspondingly, the color of AgNPs in solution changes from bright yellow to pink, and the UV-vis characteristic absorption peak of AgNPs at about 400 nm decreases; simultaneously, a new absorption band appears at about 520 nm. Irreversible inhibition of AChE activity caused by dipterex, prevents the aggregation of AgNPs. Thus, a UV-vis spectrophotometric method was developed for the analysis of dipterex. The absorbance ratio A(396nm)/A(520nm) was found to be linearly related to the concentration of dipterex in the range of 0.25-37.5 ng mL(-1) with a detection limit of 0.18 ng mL(-1). This method was used successfully to analyse dipterex in spiked, different water samples. (C) 2013 Elsevier B.V. All rights reserved.