Differential detection of Newcastle disease virus strains by degenerate primers based RT-PCR

被引:16
作者
Tiwari, AK [1 ]
Kataria, RS
Nanthakumar, T
Dash, BB
Desai, G
机构
[1] Indian Vet Res Inst, Natl Biotechnol Ctr, Izatnagar 243122, Uttar Pradesh, India
[2] Indian Vet Res Inst, Div Avian Dis, Izatnagar 243122, Uttar Pradesh, India
[3] High Secur Anim Dis Lab, Bhopal 462021, India
关键词
Newcastle disease virus; differentiation; F gene; degenerate primers; RT-PCR;
D O I
10.1016/j.cimid.2003.09.002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Degenerate primers based RT-PCR (previously described by [Avian Dis 26 (1997) 837]) has been used for the detection and differentiation of Newcastle disease (ND) viruses. Two sets of primers (A + B and A + C), with common forward primer and distinct reverse degenerate primers, designed from fusion protein gene encoding for cleavage site, could differentiate virulent and avirulent Newcastle disease viruses (NDV). Both sets of primers amplified 'F' gene sequence of virulent (velogenic and mesogenic) viruses, whereas in avirulent strains, amplification was only with primer set A + C. Total 10 NDV isolates and two clinical samples including both known and unknown pathotypes, were checked. Based on amplification results 5 viruses were found to be virulent type and 6 as avirulent with one of the two clinical samples, earlier positive by RT-PCR using non-degenerate V gene specific primers was found negative in this study. The technique has been found to be a simple and quick for the detection and differentiation of virulent and avirulent NDV, which is important for control of the disease in the events of the outbreaks. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:163 / 169
页数:7
相关论文
共 13 条
  • [1] Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1)
    Aldous, EW
    Alexander, DJ
    [J]. AVIAN PATHOLOGY, 2001, 30 (02) : 117 - 128
  • [2] Alexander D. J., 1991, Diseases of poultry., P496
  • [3] Alexander D J, 1974, Avian Pathol, V3, P269, DOI 10.1080/03079457409353840
  • [4] Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene
    BallagiPordany, A
    Wehmann, E
    Herczeg, J
    Belak, S
    Lomniczi, B
    [J]. ARCHIVES OF VIROLOGY, 1996, 141 (02) : 243 - 261
  • [5] DETECTION OF NEWCASTLE-DISEASE VIRUS-RNA IN INFECTED ALLANTOIC FLUIDS BY INVITRO ENZYMATIC AMPLIFICATION (PCR)
    JESTIN, V
    JESTIN, A
    [J]. ARCHIVES OF VIROLOGY, 1991, 118 (3-4) : 151 - 161
  • [6] Differentiation of virulent and non-virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction
    Kant, A
    Koch, G
    Van Roozelaar, DJ
    Balk, F
    Ter Huurne, A
    [J]. AVIAN PATHOLOGY, 1997, 26 (04) : 837 - 849
  • [7] PROTEOLYTIC CLEAVAGE OF VIRAL GLYCOPROTEINS AND ITS SIGNIFICANCE FOR VIRULENCE OF NEWCASTLE-DISEASE VIRUS
    NAGAI, Y
    KLENK, HD
    ROTT, R
    [J]. VIROLOGY, 1976, 72 (02) : 494 - 508
  • [8] Sequence analysis of the cleavage site-encoding region of the fusion protein gene of Newcastle disease viruses from India and Nepal
    Nanthakumar, T
    Tiwari, AK
    Kataria, RS
    Butchaiah, G
    Kataria, JM
    Goswami, PP
    [J]. AVIAN PATHOLOGY, 2000, 29 (06) : 603 - 607
  • [9] Pathotyping of Newcastle disease viruses by RT-PCR and restriction enzyme analysis
    Nanthakumar, T
    Kataria, RS
    Tiwari, AK
    Butchaiah, G
    Kataria, JM
    [J]. VETERINARY RESEARCH COMMUNICATIONS, 2000, 24 (04) : 275 - 286
  • [10] CHARACTERIZATION OF NEWCASTLE-DISEASE VIRUS ISOLATES BY REVERSE TRANSCRIPTION PCR COUPLED TO DIRECT NUCLEOTIDE SEQUENCING AND DEVELOPMENT OF SEQUENCE DATABASE FOR PATHOTYPE PREDICTION AND MOLECULAR EPIDEMIOLOGIC ANALYSIS
    SEAL, BS
    KING, DJ
    BENNETT, JD
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (10) : 2624 - 2630