In vivo circular RNA production using a constitutive promoter for high-level expression

被引：10

作者：

Umekage, So ^{[1
]}

Kikuchi, Yo ^{[1
]}

机构：

[1] Toyohashi Univ Technol, Div Life Sci & Biotechnol, Dept Ecol Engn, Aichi 4418580, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2009年 / 108卷 / 04期

关键词：

Circularization; Permuted intron-exon; Aptamer; In vivo expression; Constitutive promoter; ACTIVATED HIV-1 TRANSCRIPTION; ESCHERICHIA-COLI; INTRON; SEQUENCES; COMPLEX; GENE;

D O I：

10.1016/j.jbiosc.2009.04.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circular RNA expression method based on an induction-free vector system utilizing the highly efficient constitutive lpp promoter. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：354 / 356

页数：3

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