An improved micropropagation protocol for stone pine (Pinus pinea L.)

被引：34
|
作者：
Alonso, Pablo
Moncalean, Palorna
Fernandez, Belen
Rodriguez, Ana
Centeno, Maria Luz
Ordas, Ricardo J. [1 ]
机构：
[1] Ctr Arkaute, Inst Vasco Invest & Desarrollo, Pais Vasco 01080, Spain
[2] Univ Leon, Fac Ciencias Biol & Ambientales, Dept Biol Vegetal, Leon, Spain
[3] Univ Oviedo, Dept Biol Organismos & Sistemas, Inst Biotecnol, Area Fisiol Vegetal, Oviedo 330071, Spain
来源：
ANNALS OF FOREST SCIENCE | 2006年 / 63卷 / 08期
关键词：
conifers; micropropagation; organogenesis; rooting; tissue culture;
D O I：
10.1051/forest:2006071
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
A protocol for micropropagation from isolated cotyledons from Pinus pinea L. has been developed. Major improvements are the increase in rooting rates and the description for the first time of a successful continuous multiplication procedure. For bud induction, isolated cotyledons were cultured on 1/2 LP with 44.4 mu M BA during 4 days. Shoot development was obtained by transfer to 1/2 LP hormone-free medium with activated charcoal. Since an additional 20 weeks were required for shoot elongation and rooting, at least 70 plantlets can be expected per seed after 29 weeks based on a 70% success rate. Besides, shoots can be micropropagated successively by subculturing on 1/2 LPC.
引用
收藏
页码：879 / 885
页数：7
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