A component of green tea, (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins

被引：101
作者：
Qin, Jie
Xie, Li-Ping
Zheng, Xiang-Yi
Wang, Yun-Bin
Bai, Yu
Shen, Hua-Feng
Li, Long-Cheng
Dahiya, Rajvir
机构：
[1] Zhejiang Univ, Affiliated Hosp 1, Coll Med, Dept Urol, Hangzhou 310003, Zhejiang Prov, Peoples R China
[2] Univ Calif San Francisco, Dept Urol, San Francisco, CA 94143 USA
[3] Vet Affairs Med Ctr, San Francisco, CA 94121 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 354卷 / 04期
关键词：
bladder cancer; Akt; EGCG; apoptosis; Bcl-2; SIGNALING PATHWAY; INHIBITION; GROWTH; ACTIVATION; EXPRESSION; INDUCTION; RISK; BAX; P53;
D O I：
10.1016/j.bbrc.2007.01.003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bladder cancer is the fourth most common cancer in men and ninth most common in women. It has a protracted course of progression and is thus an ideal candidate for chemoprevention strategies and trials. This study was conducted to evaluate the chemopreventive/antiproliferative potential of (-)-epigallocatechin gallate (EGCG, the major phytochemical in green tea) against bladder cancer and its mechanism of action. Using the T24 human bladder cancer cell line, we found that EGCG treatment caused dose- and time-dependent inhibition of cellular proliferation and cell viability, and induced apoptosis. Mechanistically, EGCG inhibits phosphatidylinositol 3'-kinase/Akt activation that, in turn, results in modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells. These findings suggest that EGCG may be an important chemoprevention agent for the management of bladder cancer. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：852 / 857
页数：6
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