SIMULTANEOUS DETERMINATION OF PHILLYRIN AND FORSYTHIASIDE IN BEAGLE DOG PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH GRADIENT ELUTION AND ITS APPLICATION TO PHARMACOKINETIC STUDIES

被引：5

作者：

Li Yun-Xia ^{[1
]}

Peng Cheng ^{[1
]}

Zhang Ruo-Qi ^{[2
]}

Li Xue ^{[2
]}

Jiang Xue-Hua ^{[2
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Sichuan, Peoples R China

[2] Sichuan Univ, W China Sch Pharm, Sichuan, Peoples R China

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Beagle dog; Forsythiaside; Gradient elution; HPLC; Pharmacokinetics; Phillyrin; METHANOL EXTRACTS; HEPATIC-INJURY; CRUDE DRUGS; RAT PLASMA;

D O I：

10.1080/00032710903325765

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple HPLC method was developed to study the pharmacokinetics of phillyrin and forsythiaside in beagle dog after intravenous administration. The drugs were separated on a reversed-phase C18 column and detected at 228 and 332 nm. Good linearity was achieved in the range of 0.078 similar to 40.0 mu g . ml(-1). The intra-and inter-assay variation coefficients for this analysis were no more than 15%, respectively. The analytical recovery, sensitivity, and accuracy of this assay were adequate for characterization of the pharmacokinetics of intravenous administration of phillyrin and forsythiaside to beagle dogs and the assay has been successfully applied to provide pharmacokinetic data.

引用

页码：209 / 218

页数：10

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