High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale

被引:12
|
作者
Dai, X. G. [1 ]
Shi, X. P. [1 ]
Ye, Y. M. [1 ]
Fu, Q. [1 ]
Bao, M. Z. [1 ]
机构
[1] Huazhong Agr Univ, Minist Educ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China
关键词
AgNO3; auxin; Brassica oleracea var. acephala; cytokinin; genotype; rooting; shoot regeneration; CANOLA BRASSICA-NAPUS; INFLUENCING SHOOT REGENERATION; IN-VITRO; GENETIC-TRANSFORMATION; PERILLA-FRUTESCENS; GENOTYPE; L; MORPHOGENESIS; ETHYLENE; GROWTH;
D O I
10.1007/s10535-009-0141-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A high frequency shoot regeneration system for ornamental kale [Brassica oleracea L. var. acephala (D.C.) Alef.] was firstly established from seedling cotyledon and hypocotyl explants. The ability of cotyledon and hypocotyl to produce adventitious shoots varied depending upon genotype, seedling age and culture medium. The maximum shoot regeneration frequency was obtained when the explants from cv. Nagoya 4-d-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg dm(-3) 6-benzylaminopurine (BA) and 0.1 mg dm(-3) naphthaleneacetic acid (NAA). The frequency of shoot regeneration was 65.0 % for cotyledons, 76.1 % for hypocotyls; and the number of shoots per explant was 4.3 for cotyledons, 8.2 for hypocotyls. Hypocotyl explants were found to be more responsive for regeneration when compared with cotyledons. Among the 4 cultivars tested, Nagoya showed the best shoot regeneration response. The addition of 3.0 mg dm(-3) AgNO3 was beneficial to shoot regeneration. Roots were formed on the base of the shoots when cultured on half-strength MS medium.
引用
收藏
页码:769 / 773
页数:5
相关论文
共 50 条