Electrospun chitosan/PVA/bioglass Nanofibrous membrane with spatially designed structure for accelerating chronic wound healing

被引:115
作者
Chen, Qingchang [1 ]
Wu, Jun [2 ,3 ]
Liu, Yuan [1 ]
Li, Yanqun [1 ]
Zhang, Chuqiu [1 ]
Qi, Weichen [1 ,3 ]
Yeung, Kelvin W. K. [2 ,3 ]
Wong, Tak Man [2 ,3 ]
Zhao, Xiaoli [1 ]
Pan, Haobo [1 ]
机构
[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Res Ctr Human Tissues & Organs Degenerat, Shenzhen 518055, Peoples R China
[2] Univ Hong Kong Shenzhen Hosp, Shenzhen Key Lab Innovat Technol Orthopaed Trauma, Shenzhen, Peoples R China
[3] Univ Hong Kong, Dept Orthopaed & Traumatol, Pokfulum, 21 Sassoon Rd, Hong Kong 999077, Peoples R China
来源
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2019年 / 105卷
基金
中国国家自然科学基金;
关键词
Sequential electrospinning; Wound healing; Diabetic chronic wounds; Bioglass; Spatially designed multilayer structure; ENDOTHELIAL GROWTH-FACTOR; IN-VITRO; CHITOSAN; PH; SCAFFOLDS; HYDROGEL; ANTIOXIDANT; BIOACTIVITY; HEMOSTASIS; COLLAGEN;
D O I
10.1016/j.msec.2019.110083
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Cutaneous wounds, especially chronic wounds, remain clinical challenges, and this is partially due to the complex healing process composed of four overlapping but distinct stages including hemostasis, inflammation, proliferation and remodeling. Therefore, wound dressings with spatially designed structures which can temporally regulate certain bioactive components to function at specific healing stages might be able to accelerate the healing process. In this study, nanobioglass incorporated chitosan-PVA (polyvinyl alcohol) trilayer nanofibrous membrane (nBG-TFM) was fabricated via sequential electrospinning. This membrane exhibited excellent biocompatibility, antibacterial activity and regeneration promotion effect. Furthermore, spatially designed structure optimized functions of each component and provided more suitable microenvironment as compared with uniform membrane. Rat full-thickness skin defects model and mice diabetic chronic wound model showed that nBG-TFM could achieve significantly accelerated and enhanced healing, in terms of complete re-epithelialization, improved collagen alignment and formation of skin appendages. It was revealed that nBG-TFM functioned through upregulating growth factors including VEGF and TGF-beta. Meanwhile inflammatory cytokines such as TNF-alpha and IL-1 beta were downregulated. The technology presented in this study shed new light on designing functional wound dressings which can promote healing of chronic wounds.
引用
收藏
页数:11
相关论文
共 67 条
[1]  
Atiyeh Bishara S., 2002, Current Pharmaceutical Biotechnology, V3, P179, DOI 10.2174/1389201023378283
[2]   Reactive oxygen species and their detoxification in healing skin wounds [J].
auf dem Keller, Ulrich ;
Kuemin, Angelika ;
Braun, Susanne ;
Werner, Sabine .
JOURNAL OF INVESTIGATIVE DERMATOLOGY SYMPOSIUM PROCEEDINGS, 2006, 11 (01) :106-111
[3]   Anticancer and Anti-Inflammatory Properties of Chitin and Chitosan Oligosaccharides [J].
Azuma, Kazuo ;
Osaki, Tomohiro ;
Minami, Saburo ;
Okamoto, Yoshiharu .
JOURNAL OF FUNCTIONAL BIOMATERIALS, 2015, 6 (01) :33-49
[4]   Growth factors and cytokines in wound healing [J].
Barrientos, Stephan ;
Stojadinovic, Olivera ;
Golinko, Michael S. ;
Brem, Harold ;
Tomic-Canic, Marjana .
WOUND REPAIR AND REGENERATION, 2008, 16 (05) :585-601
[5]   Nanoparticle-Based Dressing: The Future of Wound Treatment? [J].
Berthet, Morgane ;
Gauthier, Yves ;
Lacroix, Celine ;
Verrier, Bernard ;
Monge, Claire .
TRENDS IN BIOTECHNOLOGY, 2017, 35 (08) :770-784
[6]   Advanced Therapeutic Dressings for Effective Wound HealingA Review [J].
Boateng, Joshua ;
Catanzano, Ovidio .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2015, 104 (11) :3653-3680
[7]   A novel spatially designed and functionally graded electrospun membrane for periodontal regeneration [J].
Bottino, Marco C. ;
Thomas, Vinoy ;
Janowski, Gregg M. .
ACTA BIOMATERIALIA, 2011, 7 (01) :216-224
[8]   Molecular markers in patients with chronic wounds to guide surgical debridement [J].
Brem, Harold ;
Stojadinovic, Olivera ;
Diegelmann, Robert F. ;
Entero, Hyacinth ;
Lee, Brian ;
Pastar, Irena ;
Golinko, Michael ;
Rosenberg, Harvey ;
Tomic-Canic, Marjana .
MOLECULAR MEDICINE, 2007, 13 (1-2) :30-39
[9]   PolySTAT-modified chitosan gauzes for improved hemostasis in external hemorrhage [J].
Chan, Leslie W. ;
Kim, Chae Hwa ;
Wang, Xu ;
Pun, Suzie H. ;
White, Nathan J. ;
Kim, Tae Hee .
ACTA BIOMATERIALIA, 2016, 31 :178-185
[10]   Combined effects of PLGA and vascular endothelial growth factor promote the healing of non-diabetic and diabetic wounds [J].
Chereddy, Kiran Kumar ;
Lopes, Alessandra ;
Koussoroplis, Salome ;
Payen, Valery ;
Moia, Claudia ;
Zhu, Huijun ;
Sonveaux, Pierre ;
Carmeliet, Peter ;
des Rieux, Anne ;
Vandermeulen, Gaelle ;
Preat, Veronique .
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2015, 11 (08) :1975-1984