Molecular diagnosis of the natural infection rate due to Leishmania sp in sandflies (Psychodidae, Lutzomyia) in the Amazon region of Maranhao, Brazil

被引：39
作者：
Oliveira-Pereira, Yrla Nivea
Macario Rebelo, Jose Manuel
Pinto Moraes, Jorge Luiz
Ferreira Pereira, Silma Regina
机构：
[1] Univ Fed Maranhao, Curso Pos Grad, BR-65080040 Sao Luis, MA, Brazil
[2] Univ Fed Maranhao, Entomol Lab, Dept Pathol, BR-65080040 Sao Luis, MA, Brazil
[3] Univ Fed Maranhao, Dept Biol, BR-65080040 Sao Luis, MA, Brazil
来源：
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL | 2006年 / 39卷 / 06期
关键词：
molecular biology; sandflies; Leishmania; Lutzomyia whitmani;
D O I：
10.1590/S0037-86822006000600005
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The natural infection rate due to Leishmania was studied in three different sandfly species using the polymerase chain reaction technique. Leishmania specific primers were designed to examine whether sandfly pools were infected. In total 1,100 female of Lutzomyia choti, were analyzed. Among all the pools examined, four pools of Lutzomyia whitmani were positive, but none of the pools of the other two species were infected. Thus, a total infection rate of 0.4% was established in this study. A similar infection rate was found in previous studies, suggesting that Lutzomyia whitmani transmits Leishmania to mammals in Buriticupu, Maranhao.
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收藏
页码：540 / 543
页数：4
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