Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors

被引:25
作者
Yu, Ziwei [1 ]
Men, Yongzhi [1 ]
Dong, Pin [1 ]
机构
[1] Shanghai Jiao Tong Univ, Dept Otolaryngol, Shanghai Gen Hosp, Sch Med, 100 ilaining Rd,Bldg 2,9th Floor, Shanghai 200080, Peoples R China
关键词
neural stem cells; schwann cells; co-culture; neurotropic factors; PERIPHERAL-NERVE REPAIR; TRANSECTED SPINAL-CORD; AXONAL REGENERATION; FUNCTIONAL RECOVERY; SCIATIC-NERVE; TRANSPLANTATION; INJURY; EXPRESSION; TISSUE; BRAIN;
D O I
10.3892/etm.2017.4183
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The present study investigated whether co-culturing Schwann cells (SCs) with neural stem cells (NSCs) improves viability, direction of differentiation and secretion of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in NSCs. The three groups assessed were as follows: SCs, NSCs, and a co-culture of SCs and NSCs. Cellular morphological changes were observed under an inverted phase contrast microscope and quantified. Cells were identified by immunofluorescence staining: S100 for SCs, Nestin for NSCs, microtubule associated protein (Map) 2 and NeuN for neurons and glial fibrillary acidic protein for astrocytes. Cell viability was evaluated by MTT assay. Secretion of BDNF and GDNF was quantified; mRNA expression was quantified by reverse transcription-quantitative polymerase chain reaction. The majority of NSCs in the co-cultured group differentiated into neurons. The cell survival rate of the co-culture group was significantly higher than the other groups on days 3, 5 and 10 (P<0.01). The secretion of BDNF in the co-culture group was significantly higher than NSCs on days 3, 5 and 7 (P<0.05), while the amount of GDNF in co-culture was significantly higher than both NSCs and SCs on day 1 (P<0.05). BDNF and GDNF gene expression in the co-culture group was significantly higher than SCs (P<0.01). Gene expression of Map2 in co-culture group was also significantly higher than both NSC and SC groups (P<0.01). Therefore, co-cultured SCs and NSCs promote differentiation of NSCs into neurons and secrete higher levels of neurotropic factors including BDNF and GDNF.
引用
收藏
页码:2029 / 2035
页数:7
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