Identification of nucleated red blood cells in maternal circulation: A second step in screening for fetal aneuploidies and pregnancy complications

被引:43
作者
Mavrou, A. [1 ]
Kouvidi, E. [1 ]
Antsaklis, A. [1 ]
Souka, A. [1 ]
Tzeli, S. Kitsiou [1 ]
Kolialexi, A. [1 ]
机构
[1] Univ Athens, Sch Med, Dept Obstet & Gynecol 1, GR-11527 Athens, Greece
关键词
fetal cells; non-invasive prenatal diagnosis; fetal aneuploidies; screening test;
D O I
10.1002/pd.1640
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background Identification of fetal nucleated red blood cells (NRBCs) in maternal circulation can facilitate non-invasive prenatal diagnosis, but technical difficulties still exist. An increase in the number of circulating NRBCs, however, could indicate fetal aneuploidies or pregnancy complications. Materials and Methods The number of NRBCs was determined from 20 mL peripheral blood in 351 women in the second trimester of pregnancy after isolation by magnetic cell sorting (MACS) with anti-CD71 antibody and identification with May-Grunwald/Giemsa staining. Results An average of eight NRBCs (range 1-12) were identified among 282 women with chromosomally normal fetuses. In cases known to carry aneuploid fetuses the mean number was 35 (range 7-113), but when the fetus had trisomy 21 (n = 17) an average of 71 NRBCs were identified. Among 26 carriers of beta-thalassemia, 42 NRBCs (range 22-158) were isolated. In pregnancies with abnormal Doppler findings in both uterine arteries (n = 20), 15 NRBCs (range 2-75) were isolated. Conclusion Determining the number of NRBCs in maternal circulation could represent an additional screening step for fetal aneuploidies, as long as the anemic status of the mother is taken into consideration. However, more cases with abnormal Doppler results must be investigated before this test is used for in the prediction of pregnancy complications. Copyright (C) 2006 John Wiley & Sons, Ltd.
引用
收藏
页码:150 / 153
页数:4
相关论文
共 28 条
[1]   Increased fetal erythroblasts in women who subsequently develop pre-eclampsia [J].
Al-Mufti, R ;
Hambley, H ;
Albaiges, G ;
Lees, C ;
Nicolaides, KH .
HUMAN REPRODUCTION, 2000, 15 (07) :1624-1628
[2]  
Al-Mufti R, 1999, AM J MED GENET, V85, P66, DOI 10.1002/(SICI)1096-8628(19990702)85:1<66::AID-AJMG11>3.0.CO
[3]  
2-2
[4]   Fetal cells in maternal blood of pregnancies with severe fetal growth restriction [J].
Al-Mufti, R ;
Lees, C ;
Albaiges, G ;
Hambley, H ;
Nicolaides, KH .
HUMAN REPRODUCTION, 2000, 15 (01) :218-221
[5]   Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and after pregnancy: implications for noninvasive prenatal diagnosis [J].
Ariga, H ;
Ohto, H ;
Busch, MP ;
Imamura, S ;
Watson, R ;
Reed, W ;
Lee, TH .
TRANSFUSION, 2001, 41 (12) :1524-1530
[6]  
Babochkina T, 2005, HAEMATOLOGICA, V90, P740
[7]  
BIANCHI DW, 1992, HUM GENET, V90, P368
[8]   Fetal gender and aneuploidy detection using fetal cells in maternal blood: analysis of NIFTY I data [J].
Bianchi, DW ;
Simpson, JL ;
Jackson, LG ;
Elias, S ;
Holzgreve, W ;
Evans, MI ;
Dukes, KA ;
Sullivan, LM ;
Klinger, KW ;
Bischoff, FZ ;
Hahn, S ;
Johnson, KL ;
Lewis, D ;
Wapner, RJ .
PRENATAL DIAGNOSIS, 2002, 22 (07) :609-615
[9]   PCR quantitation of fetal cells in maternal blood in normal and aneuploid pregnancies [J].
Bianchi, DW ;
Williams, JM ;
Sullivan, LM ;
Hanson, FW ;
Klinger, KW ;
Shuber, AP .
AMERICAN JOURNAL OF HUMAN GENETICS, 1997, 61 (04) :822-829
[10]   DETECTION OF 47,XYY TROPHOBLAST FETAL CELLS IN MATERNAL BLOOD BY FLUORESCENCE INSITU HYBRIDIZATION AFTER USING IMMUNOMAGNETIC LYMPHOCYTE DEPLETION AND FLOW-CYTOMETRY SORTING [J].
CACHEUX, V ;
MILESIFLUET, C ;
TACHDJIAN, G ;
DRUART, L ;
BRUCH, JF ;
HSI, BL ;
UZAN, S ;
NESSMANN, C .
FETAL DIAGNOSIS AND THERAPY, 1992, 7 (3-4) :190-194