Controlled Manipulation of Bacteriophages Using Single-Virus Force Spectroscopy

被引：18

作者：

Alsteens, David ^{[2
]}

Pesavento, Emanuele ^{[1
]}

Cheuvart, Gilles ^{[2
]}

Dupres, Vincent ^{[2
]}

Trabelsi, Heykel ^{[1
]}

Soumillion, Patrice ^{[1
]}

Dufrene, Yves F. ^{[2
]}

机构：

[1] Univ Catholique Louvain, Inst Sci Vie, Lab Ingn Prot & Peptides, B-1348 Louvain, Belgium

[2] Univ Catholique Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium

来源：

ACS NANO | 2009年 / 3卷 / 10期

关键词：

phage display; bacteriophages; single virus manipulation; atomic force microscopy; force spectroscopy; ANTIGEN-BINDING FORCES; ENERGY LANDSCAPES; HUMAN RHINOVIRUS; LIVE CELLS; MICROSCOPY; PHAGE; RESOLUTION; NANOWIRES; MOLECULES; COMPLEX;

D O I：

10.1021/nn900778t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method is described for the site-directed manipulation of single filamentous bacteriophages, by using phage display technology and atomic force microscopy. f1 filamentous bacteriophages were genetically engineered to display His-tags on their pIX tail. Following adsorption on nitrilotriacetate-terminated surfaces, force spectroscopy with tips bearing monoclonal anti-PIII antibodies was used to pull on individual phages via their PIII head. Analysis of the force-extension profiles revealed that upon pulling, the phages are progressively straightened into an extended orientation until rupture of the anti-PIII/PIII complex. The single-virus manipulation technique presented here provides new opportunities for understanding the forces driving cell-virus and material-virus interactions, and for characterizing the binding properties of polypeptide sequences or proteins selected by the phage display technology.

引用

页码：3063 / 3068

页数：6

共 33 条

[1] Unfolding Individual Als5p Adhesion Proteins on Live Cells [J].

Alsteens, David ;

Dupres, Vincent ;

Klotz, Stephen A. ;

Gaur, Nand K. ;

Lipke, Peter N. ;

Dufrene, Yves F. .

ACS NANO, 2009, 3 (07) :1677-1682

[2] OPTICAL TRAPPING AND MANIPULATION OF VIRUSES AND BACTERIA [J].

ASHKIN, A ;

DZIEDZIC, JM .

SCIENCE, 1987, 235 (4795) :1517-1520

[3] Antigen binding forces of single antilysozyme Fv fragments explored by atomic force microscopy [J].

Berquand, A ;

Xia, N ;

Castner, DG ;

Clare, BH ;

Abbott, NL ;

Dupres, V ;

Adriaensen, Y ;

Dufrêne, YF .

LANGMUIR, 2005, 21 (12) :5517-5523

[4] A new, simple method for linking of antibodies to atomic force microscopy tips [J].

Ebner, Andreas ;

Wildling, Linda ;

Kamruzzahan, A. S. M. ;

Rankl, Christian ;

Wruss, Juergen ;

Hahn, Christoph D. ;

Hoelzl, Martin ;

Zhu, Rong ;

Kienberger, Ferry ;

Blaas, Dieter ;

Hinterdorfer, Peter ;

Gruber, Hermann J. .

BIOCONJUGATE CHEMISTRY, 2007, 18 (04) :1176-1184

[5] Dynamic strength of molecular adhesion bonds [J].

Evans, E ;

Ritchie, K .

BIOPHYSICAL JOURNAL, 1997, 72 (04) :1541-1555

[6] Bacteriophage capsids: Tough nanoshells with complex elastic properties [J].

Ivanovska, IL ;

de Pablo, PJ ;

Ibarra, B ;

Sgalari, G ;

MacKintosh, FC ;

Carrascosa, JL ;

Schmidt, CF ;

Wuite, GJL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7600-7605

[7] Filamentous phage display in the new millennium [J].

Kehoe, JW ;

Kay, BK .

CHEMICAL REVIEWS, 2005, 105 (11) :4056-4072

[8] Single M13 bacteriophage tethering and stretching [J].

Khalil, Ahmad S. ;

Ferrer, Jorge M. ;

Brau, Ricardo R. ;

Kottmann, Stephen T. ;

Noren, Christopher J. ;

Lang, Matthew J. ;

Belcher, Angela M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (12) :4892-4897

[9] Visualization of single receptor molecules bound to human rhinovirus under physiological conditions [J].

Kienberger, F ;

Rankl, C ;

Pastushenko, V ;

Zhu, R ;

Blaas, D ;

Hinterdorfer, P .

STRUCTURE, 2005, 13 (09) :1247-1253

[10] Imaging of viruses by atomic force microscopy [J].

Kuznetsov, YG ;

Malkin, AJ ;

Lucas, RW ;

Plomp, M ;

McPherson, A .

JOURNAL OF GENERAL VIROLOGY, 2001, 82 :2025-2034

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