Optimization of blood handling for plasma extracellular vesicle isolation

被引：4

作者：

Kabagwira, Janviere ^{[1
]}

Gonda, Amber ^{[1
]}

Vallejos, Paul ^{[1
]}

Babcock, Blake ^{[2
]}

Langridge, William H. R. ^{[1
]}

Senthil, Maheswari ^{[3
]}

Wall, Nathan R. ^{[1
,4
]}

机构：

[1] Loma Linda Univ, Dept Basic Sci, Div Biochem, Loma Linda, CA 92350 USA

[2] Loma Linda Univ Hlth, Div Surg Oncol, Loma Linda, CA USA

[3] Univ Calif Irvine, Div Surg Oncol, 101 City Dr South,Bldg 55, Orange, CA 92868 USA

[4] Loma Linda Univ, Ctr Hlth Dispar & Mol Med, 11085 Campus St,Mortensen Hall 160, Loma Linda, CA 92350 USA

来源：

JOURNAL OF NANOPARTICLE RESEARCH | 2021年 / 23卷 / 03期

关键词：

Extracellular vesicle; Liquid biopsy; Blood handling; Proteins; Biodepositories;

D O I：

10.1007/s11051-021-05169-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Extracellular vesicles (EVs) are emerging targets for biomarker discovery. However, suitable clinical collection and storage methods for blood plasma-derived EVs have not been fully described. We investigated the long- and short-term effects of temperature and time on the plasma-derived EV concentration, size, protein, and RNA content. EVs isolated from freshly collected human blood stored for various periods of time at either room temperature (RT) or 4 degrees C were analyzed for concentration, size, and protein content. In addition, EVs isolated from plasma stored at -80 degrees C were compared to frozen EV pellets. We found that the number, size, protein, and RNA content of EVs were similar between blood samples stored at RT for 24h compared to samples processed shortly after collection or stored at 4 degrees C vs. -80 degrees C. It is therefore feasible to isolate and analyze comparable, stable EVs from blood plasma stored for varying periods of time and at various temperatures.

引用

页数：11

共 38 条

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