The protective role of silymarin against methotrexate-induced gastrointestinal injury

被引：1

作者：

Corum, Duygu Durna ^{[1
,2
]}

Kart, Asim ^{[1
]}

机构：

[1] Mehmet Akif Ersoy Univ, Dept Pharmacol & Toxicol, Fac Vet, Burdur, Turkey

[2] Kastamonu Univ, Arac Rafet Vergili Vocat Sch, Kastamonu, Turkey

来源：

INDIAN JOURNAL OF ANIMAL RESEARCH | 2017年 / 51卷 / 02期

关键词：

GI injury; GSH; MDA; Methotrexate; Sialic acid; Silymarin; OXIDATIVE STRESS; SIALIC-ACID; ANTIOXIDANT; TOXICITY; ENZYMES; SILYBIN;

D O I：

10.18805/ijar.10767

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The protective effect of silymarin was investigated against methotrexate-induced GI injury. Forty Swiss-Albino mice were divided into 4 groups as control (Normal saline), methotrexate (15 mg/kg), silymarin (100 mg/kg) and methotrexate+silymarin (100 mg/kg + 15 mg/kg respectively). Blood and tissue samples were collected from the animals for biochemical and pathological examinations. Plasma, intestinal and gastric tissue malondialdehyde and TSA levels in methotrexate group were significantly higher than in control whereas whole blood glutathione concentration was lower in methotraxe group than in control. Plasma, intestinal and gastric TSA levels in methotrexate group were significantly increased compared to control and methotrexate+silymarin groups. Gastric tissue of methotrexate treated mice showed degeneration, necrosis, desquamation and widespread edema. However, these alterations were less severe in methotrexate+silymarin group. In conclusion, silymarin could be of therapeutic value against methotrexate-induced GI injury.

引用

页码：401 / 405

页数：5

共 25 条

[1]

Asghar Z, 2008, PAK J PHARM SCI, V21, P249

[2] Differential effects of silymarin and its active component silibinin on plasma membrane stability and hepatocellular lysis [J].

Basiglio, Cecilia L. ;

Sanchez Pozzi, Enrique J. ;

Mottino, Aldo D. ;

Roma, Marcelo G. .

CHEMICO-BIOLOGICAL INTERACTIONS, 2009, 179 (2-3) :297-303

[3]

Beutler E., 1986, J LAB CLIN MED, V28, P882

[4] Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes.: Part I.: Silymarin and its flavonolignans [J].

Chlopcíková, A ;

Psotová, J ;

Miketová, P ;

Simánek, V .

PHYTOTHERAPY RESEARCH, 2004, 18 (02) :107-110

[5] Intervention of α-lipoic acid ameliorates methotrexate-induced oxidative stress and genotoxicity: A study in rat intestine [J].

Dadhania, V. P. ;

Tripathi, D. N. ;

Vikram, A. ;

Ramarao, P. ;

Jena, G. B. .

CHEMICO-BIOLOGICAL INTERACTIONS, 2010, 183 (01) :85-97

[6] Methotrexate causes oxidative stress in rat kidney tissues [J].

Devrim, E ;

Çetin, R ;

Kiliçoglu, B ;

Ergüder, BI ;

Avci, A ;

Durak, I .

RENAL FAILURE, 2005, 27 (06) :771-773

[7] Silybin and silymarin -: New and emerging applications in medicine [J].

Gazak, Radek ;

Walterova, Daniela ;

Kren, Vladimir .

CURRENT MEDICINAL CHEMISTRY, 2007, 14 (03) :315-338

[8] Amelioration of methotrexate-induced enteritis by melatonin in rats [J].

Jahovic, N ;

Sener, G ;

Çevik, H ;

Ersoy, Y ;

Arbak, S ;

Yegen, BÇ .

CELL BIOCHEMISTRY AND FUNCTION, 2004, 22 (03) :169-178

[9] THE PHARMACOLOGY AND CLINICAL USE OF METHOTREXATE [J].

JOLIVET, J ;

COWAN, KH ;

CURT, GA ;

CLENDENINN, NJ ;

CHABNER, BA .

NEW ENGLAND JOURNAL OF MEDICINE, 1983, 309 (18) :1094-1104

[10] Local prevention of oxidative stress in the intestinal epithelium of the rat by adhesive liposomes of superoxide dismutase and tempamine [J].

Jubeh, Tareq Taha ;

Antler, Sivan ;

Haupt, Susan ;

barenholz, Yechezkel ;

Rubinstein, Abraham .

MOLECULAR PHARMACEUTICS, 2005, 2 (01) :2-11

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