Genes from Debaryomyces hansenii increase salt tolerance in Saccharomyces cerevisiae W303

被引：0
|
作者：
Prista, C
Soeiro, A
Vesely, P
Almagro, A
Ramos, J
Loureiro-Dias, MC [1 ]
机构：
[1] Inst Super Agron, Dept Bot & Engn Biol, P-1349017 Lisbon, Portugal
[2] Escuela Tecn Super Ingn Agron & Montes, Dept Microbiol, E-14071 Cordoba, Spain
来源：
FEMS YEAST RESEARCH | 2002年 / 2卷 / 02期
关键词：
Debaryomyces hansenii; salt tolerance; yeast transformant; genomic library; K+ transport;
D O I：
10.1016/S1567-1356(02)00095-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The yeast Debaryomyces hansenii has been chosen as a model for molecular studies of tolerance to NaCl. A gene library was built and transformants of Saccharomyces cerevisiae W303 containing genes from D. hansenii were selected for their ability to grow in the presence of high concentrations of NaCl and/or low concentrations of KCl. In three of these transformants 500 mM NaCl improved growth at pH 7.6 like in D. hansenii but not in S. cerevisiae. One of the plasmids restored growth at 50 muM KCl and K+ uptake in a mutant of S. cerevisiae lacking genes that encode K+ transporters. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：151 / 157
页数：7
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