Use of linear and multiple antigenic peptides in the immunodiagnosis of acute hepatitis A virus infection

被引：36
作者：
Gómara, MJ
Riedemann, S
Vega, I
Ibarra, H
Ercilla, G
Haro, I
机构：
[1] CSIC, IIQAB, Dept Quim Peptids & Prot, ES-08034 Barcelona, Spain
[2] Univ Austral Chile, Inst Med & Microbiol, Valdivia, Chile
[3] Hosp Clin Barcelona, IDIBAPS, Serv Immunol, Barcelona, Spain
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2000年 / 234卷 / 1-2期
关键词：
hepatitis A; immunodiagnosis; ELISA; synthetic peptides; multiple antigenic peptides; lipopeptides;
D O I：
10.1016/S0022-1759(99)00196-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The reactivities of two panels of anti-HAV human sera from geographically distinct areas (Chile and Spain) to synthetic peptides from the VP1, VP2 and VP3 hepatitis A virus capsid proteins were examined by an enzyme-linked immunosorbent assay (ELISA) procedure. Two and four branched multiple antigenic peptides (MAPs) and palmitoylated peptides were compared with free synthetic sequences for the detection of IgM anti-HAV antibodies in the two panels of human sera. Our results showed that acute hepatitis A patient sera recognized preferentially homogeneous two branched MAPs and palmitic acid conjugated peptides. The palmitoyl-derived VP3(110-121) peptide and the corresponding dimeric MAP were the most sensitive and appropriate for serological studies of HAV-infected patients by ELISA, sensitivity and specificity being higher than 90% and 95%, respectively. These peptide-based tests open up new avenues in the development of peptide-based immunosorbent assays for the detection of acute HAV disease. (C) 2000 Elsevier Science B.V. All rights reserved.
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收藏
页码：23 / 34
页数：12
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