Atomic Force Microscopy for Protein Detection and Their Physicochemical Characterization

被引：57

作者：

Pleshakova, Tatyana O. ^{[1
]}

Bukharina, Natalia S. ^{[1
]}

Archakov, Alexander I. ^{[1
]}

Ivanov, Yuri D. ^{[1
]}

机构：

[1] Inst Biomed Chem, 10 Pogodinskaya St, Moscow 119121, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

atomic force microscopy; protein fishing; protein characterization; LANGMUIR-BLODGETT-FILMS; SINGLE-MOLECULE; BIOMOLECULAR INTERACTIONS; MONOCLONAL-ANTIBODIES; DIRECT VISUALIZATION; ELECTRON-MICROSCOPY; PURPLE MEMBRANES; HIGH-RESOLUTION; DNA-MOLECULES; AFM;

D O I：

10.3390/ijms19041142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review is focused on the atomic force microscopy (AFM) capabilities to study the properties of protein biomolecules and to detect the proteins in solution. The possibilities of application of a wide range of measuring techniques and modes for visualization of proteins, determination of their stoichiometric characteristics and physicochemical properties, are analyzed. Particular attention is paid to the use of AFM as a molecular detector for detection of proteins in solutions at low concentrations, and also for determination of functional properties of single biomolecules, including the activity of individual molecules of enzymes. Prospects for the development of AFM in combination with other methods for studying biomacromolecules are discussed.

引用

页数：18

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