HPLC-HRMS Global Metabolomics Approach for the Diagnosis of "Olive Quick Decline Syndrome" Markers in Olive Trees Leaves

被引:8
作者
Asteggiano, Alberto [1 ]
Franceschi, Pietro [2 ]
Zorzi, Michael [1 ]
Aigotti, Riccardo [1 ]
Dal Bello, Federica [1 ]
Baldassarre, Francesca [3 ,4 ]
Lops, Francesco [5 ]
Carlucci, Antonia [5 ]
Medana, Claudio [1 ]
Ciccarella, Giuseppe [3 ,4 ]
机构
[1] Univ Torino, Dept Mol Biotechnol & Hlth Sci, Via Pietro Giuria 5, I-10125 Turin, Italy
[2] IASMA Res & Innovat Ctr, Unit Computat Biol, Fdn Edmund Mach Via E Mach 1, I-38010 San Michele All Adige, Italy
[3] Lecce Univ Salento, Biol & Environm Sci Dept, UdR INSTM, Via Monteroni, I-73100 Lecce, Italy
[4] CNR, Inst Nanotechnol, NANOTEC, Via Monteroni, I-73100 Lecce, Italy
[5] Univ Foggia, Dept Agr Sci Food & Environm, Via Napoli 25, I-71122 Foggia, Italy
关键词
olive quick decline syndrome; liquid chromatography; high-resolution mass spectrometry; metabolomics; XYLELLA-FASTIDIOSA; MASS-SPECTROMETRY; OLEANDER; DISEASE; APULIA; MS;
D O I
10.3390/metabo11010040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Olive quick decline syndrome (OQDS) is a multifactorial disease affecting olive plants. The onset of this economically devastating disease has been associated with a Gram-negative plant pathogen called Xylella fastidiosa (Xf). Liquid chromatography separation coupled to high-resolution mass spectrometry detection is one the most widely applied technologies in metabolomics, as it provides a blend of rapid, sensitive, and selective qualitative and quantitative analyses with the ability to identify metabolites. The purpose of this work is the development of a global metabolomics mass spectrometry assay able to identify OQDS molecular markers that could discriminate between healthy (HP) and infected (OP) olive tree leaves. Results obtained via multivariate analysis through an HPLC-ESI HRMS platform (LTQ-Orbitrap from Thermo Scientific) show a clear separation between HP and OP samples. Among the differentially expressed metabolites, 18 different organic compounds highly expressed in the OP group were annotated; results obtained by this metabolomic approach could be used as a fast and reliable method for the biochemical characterization of OQDS and to develop targeted MS approaches for OQDS detection by foliage analysis.
引用
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页码:1 / 15
页数:15
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