Molecular mapping of quantitative trait loci for zinc, iron and protein content in the grains of hexaploid wheat

被引:54
作者
Tiwari, Chhavi [1 ]
Wallwork, Hugh [2 ]
Arun, Balasubramaniam [1 ]
Mishra, Vinod Kumar [1 ]
Velu, Govindan [3 ]
Stangoulis, James [4 ]
Kumar, Uttam [3 ]
Joshi, Arun Kumar [1 ,5 ]
机构
[1] Banaras Hindu Univ, Inst Agr Sci, Dept Genet & Plant Breeding, Varanasi 221005, Uttar Pradesh, India
[2] South Australian Res & Dev Inst, Adelaide, SA 5001, Australia
[3] Int Maize & Wheat Improvement Ctr CIMMYT, Mexico City 06600, DF, Mexico
[4] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia
[5] South Asia Reg Off, CIMMYT, Kathmandu, Nepal
关键词
Triticum aestivum; Hexaploid wheat; QTL; X-ray fluorescence; Micronutrients; Protein; Iron and zinc content; HUMAN-NUTRITION; FOOD CROPS; IDENTIFICATION; ENVIRONMENTS; TOLERANCE; DENSITY; QTLS;
D O I
10.1007/s10681-015-1544-7
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Understanding the genetic basis of micronutrient concentration in wheat grain may provide useful information to breed for biofortified varieties through marker assisted selection (MAS). One hundred and thirty eight doubled haploid progeny of a cross between the wheat cultivars 'Berkut' and 'Krichauff' were evaluated for 2 years at two locations on the eastern Gangetic plains of India under timely (November) sown conditions. Grains were evaluated for Zn and Fe concentrations by energy-dispersive X-ray fluorescence. Using composite interval mapping, three QTLs were identified; two for Zn (1B and 2B) with a QTL (2B) co-located for Fe and the third (1A), for protein. The QTL located on chromosome 1B (flanked by wmc036-cfa2129) and 2B (flanked by gwm120-wpt2430) for Zn explained up to 23.1 and 35.9 % of mean phenotypic variation respectively, whereas up to 22.2 % was explained by the Fe QTL co-located with the Zn QTL on chromosome 2B. A QTL for grain protein was detected on chromosome 1A and flanked by the markers, wpt9592 and GBM1153 which explained up to 17.7 % of the total phenotypic variation. With their detection over consecutive seasons, the detected QTLs appeared robust and useful for MAS.
引用
收藏
页码:563 / 570
页数:8
相关论文
共 38 条
[1]   Analysis of sequence, map position, and gene expression reveals conserved essential genes for iron uptake in Arabidopsis and tomato [J].
Bauer, P ;
Thiel, T ;
Klatte, M ;
Bereczky, Z ;
Brumbarova, T ;
Hell, R ;
Grosse, I .
PLANT PHYSIOLOGY, 2004, 136 (04) :4169-4183
[2]   Detection of grain protein content QTLs across environments in tetraploid wheats [J].
Blanco, A ;
Pasqualone, A ;
Troccoli, A ;
Di Fonzo, N ;
Simeone, R .
PLANT MOLECULAR BIOLOGY, 2002, 48 (05) :615-623
[3]   The potential of genetically modified food crops to improve human nutrition in developing countries [J].
Bouis, Howarth E. .
JOURNAL OF DEVELOPMENT STUDIES, 2007, 43 (01) :79-96
[4]   Triticum dicoccoides:: An important genetic resource for increasing zinc and iron concentration in modern cultivated wheat [J].
Cakmak, I ;
Torun, A ;
Millet, E ;
Feldman, M ;
Fahima, T ;
Korol, A ;
Nevo, E ;
Braun, HJ ;
Özkan, H .
SOIL SCIENCE AND PLANT NUTRITION, 2004, 50 (07) :1047-1054
[5]   Plant nutrition research: Priorities to meet human needs for food in sustainable ways [J].
Cakmak, I .
PLANT AND SOIL, 2002, 247 (01) :3-24
[6]   Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations [J].
Distelfeld, Assaf ;
Cakmak, Ismail ;
Peleg, Zvi ;
Ozturk, Levent ;
Yazici, Atilla M. ;
Budak, Hikmet ;
Saranga, Yehoshua ;
Fahima, Tzion .
PHYSIOLOGIA PLANTARUM, 2007, 129 (03) :635-643
[7]   Quantitative trait loci analysis of zinc efficiency and grain zinc concentration in wheat using whole genome average interval mapping [J].
Genc, Y. ;
Verbyla, A. P. ;
Torun, A. A. ;
Cakmak, I. ;
Willsmore, K. ;
Wallwork, H. ;
McDonald, G. K. .
PLANT AND SOIL, 2009, 314 (1-2) :49-66
[8]   Sodium exclusion QTL associated with improved seedling growth in bread wheat under salinity stress [J].
Genc, Y. ;
Oldach, K. ;
Verbyla, A. P. ;
Lott, G. ;
Hassan, M. ;
Tester, M. ;
Wallwork, H. ;
McDonald, G. K. .
THEORETICAL AND APPLIED GENETICS, 2010, 121 (05) :877-894
[9]   Nutritious subsistence food systems [J].
Graham, Robin D. ;
Welch, Ross M. ;
Saunders, David A. ;
Ortiz-Monasterio, Ivan ;
Bouis, Howarth E. ;
Bonierbale, Merideth ;
de Haan, Stef ;
Burgos, Gabriella ;
Thiele, Graham ;
Liria, Reyna ;
Meisner, Craig A. ;
Beebe, Steve E. ;
Potts, Michael J. ;
Kadian, Mohinder ;
Hobbs, Peter R. ;
Gupta, Raj K. ;
Twomlow, Steve .
ADVANCES IN AGRONOMY, VOL 92, 2007, 92 :1-74
[10]   Marker-assisted wheat breeding: present status and future possibilities [J].
Gupta, P. K. ;
Langridge, Peter ;
Mir, R. R. .
MOLECULAR BREEDING, 2010, 26 (02) :145-161