Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device

被引：22

作者：

Bates, Steven R. ^{[1
,2
]}

Quake, Stephen R. ^{[1
,2
,3
]}

机构：

[1] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[2] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

来源：

APPLIED PHYSICS LETTERS | 2009年 / 95卷 / 07期

关键词：

biochemistry; bioMEMS; dissociation; macromolecules; microfluidics; micro-optomechanical devices; molecular biophysics; proteins; reaction rate constants; PROTEIN-INTERACTION; SPR;

D O I：

10.1063/1.3211382

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association and dissociation curves for the interactions of 6-His and T7 epitope tags with their antibodies, from which we determined the off rates, on rates, and dissociation constants.

引用

页数：3

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