Separation of fructooligosaccharides on a cation-exchange HPLC column in silver form with refractometric detection

被引：19

作者：

Antosová, M ^{[1
]}

Polakovic, M ^{[1
]}

Báles, V ^{[1
]}

机构：

[1] Slovak Univ Technol Bratislava, Fac Chem Technol, Dept Chem & Biochem Engn, Bratislava 81237, Slovakia

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 12期

关键词：

fructooligosaccharides; high performance liquid chromatography; in situ hydrolysis;

D O I：

10.1023/A:1008986426849

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic HPLC method was developed for the analysis of fructooligosaccharides in mixtures with sucrose, glucose and fructose. Fructooligosaccharides and sucrose were extensively hydrolyzed during the analyses at 80 degrees C but at 40 degrees C there was a negligible hydrolysis with a peak resolution over 1.5 for every pair of subsequently eluting saccharides.

引用

页码：889 / 892

页数：4