Separation of fructooligosaccharides on a cation-exchange HPLC column in silver form with refractometric detection

被引：19
|
作者：
Antosová, M [1 ]
Polakovic, M [1 ]
Báles, V [1 ]
机构：
[1] Slovak Univ Technol Bratislava, Fac Chem Technol, Dept Chem & Biochem Engn, Bratislava 81237, Slovakia
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 12期
关键词：
fructooligosaccharides; high performance liquid chromatography; in situ hydrolysis;
D O I：
10.1023/A:1008986426849
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An isocratic HPLC method was developed for the analysis of fructooligosaccharides in mixtures with sucrose, glucose and fructose. Fructooligosaccharides and sucrose were extensively hydrolyzed during the analyses at 80 degrees C but at 40 degrees C there was a negligible hydrolysis with a peak resolution over 1.5 for every pair of subsequently eluting saccharides.
引用
收藏
页码：889 / 892
页数：4
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