Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots

被引：23

作者：

Sylak-Glassman, Emily J. ^{[1
,2
]}

Zaks, Julia ^{[2
,3
]}

Amarnath, Kapil ^{[1
,2
]}

Leuenberger, Michelle ^{[1
,2
]}

Fleming, Graham R. ^{[1
,2
,3
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Grad Program Appl Sci & Technol, Berkeley, CA 94720 USA

来源：

PHOTOSYNTHESIS RESEARCH | 2016年 / 127卷 / 01期

基金：

美国国家科学基金会;

关键词：

Arabidopsis thaliana; Chlorophyll; Fluorescence lifetime; Fluorescence yield; Non-photochemical quenching; PHOTOSYSTEM-II; CHLOROPHYLL FLUORESCENCE; ARABIDOPSIS LEAVES; ENERGY-DISSIPATION; IN-VIVO; LIGHT; PHOTOSYNTHESIS; HETEROGENEITY; ACCLIMATION; ABSORPTION;

D O I：

10.1007/s11120-015-0104-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We describe a technique to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We show how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.

引用

页码：69 / 76

页数：8

共 27 条

[21] DETERMINATION OF ACCURATE EXTINCTION COEFFICIENTS AND SIMULTANEOUS-EQUATIONS FOR ASSAYING CHLOROPHYLL-A AND CHLOROPHYLL-B EXTRACTED WITH 4 DIFFERENT SOLVENTS - VERIFICATION OF THE CONCENTRATION OF CHLOROPHYLL STANDARDS BY ATOMIC-ABSORPTION SPECTROSCOPY [J].