Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices

被引：28

作者：

Small, DL ^{[1
]}

Poulter, MO ^{[1
]}

Buchan, AM ^{[1
]}

Morley, P ^{[1
]}

机构：

[1] FOOTHILLS PROV GEN HOSP,CALGARY,AB T2N 2T9,CANADA

来源：

NEUROSCIENCE LETTERS | 1997年 / 232卷 / 02期

关键词：

in vitro ischemia; excitotoxicity; reverse transcription-polymerase chain reaction; N-Methyl-D-aspartate receptor; hippocampus;

D O I：

10.1016/S0304-3940(97)00592-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-D-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. Ninety minutes after re-oxygenation and return to 10 mM glucose, there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole, as well as in the selectively vulnerable CA1 region and the resistant CA3 and dentate gyrus regions. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：87 / 90

页数：4

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