Microextraction techniques in breath biomarker analysis

Breath volatile organic compound analysis may open a non-invasive window onto (patho)physiological and metabolic processes in the body. Breath tests require controlled sampling with respect to different breath phases and on-site and point-of-care applicability. Microextraction techniques such as solid phase microextraction (SPME) or needle-trap microextraction (NTME) meet these requirements. Small sample volumes and fast and controlled sample preparation combine on-site sampling and pre-concentration in one step. Detection limits in the low ppbV range and fast and simple processing facilitate the application of distribution-based SPME for screening and targeted analysis. Exhaustive NTME has shown further advantages such as fast and automated sampling, improved stability and reproducibility with improved detection limits. Combinations of different sorbents and thermal expansion desorption have shown most promising properties when applied to water saturated breath samples. This article addresses major challenges and advantages of microextraction techniques in breath analysis. Important progress, current applications and future trends are discussed.