Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma

被引：63
作者：
Carlucci, Giuseppe [1 ]
Carney, Brandon [1 ,2 ,3 ]
Brand, Christian [1 ]
Kossatz, Susanne [1 ]
Irwin, Christopher P. [1 ]
Carlin, Sean D. [1 ]
Keliher, Edmund J. [4 ]
Weber, Wolfgang [1 ,5 ]
Reiner, Thomas [1 ,5 ]
机构：
[1] Mem Sloan Kettering Canc Ctr, Dept Radiol, New York, NY 10065 USA
[2] CUNY Hunter Coll, Dept Chem & Biochem, New York, NY 10065 USA
[3] CUNY, Grad Ctr, PhD Program Chem, New York, NY 10018 USA
[4] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA
[5] Weill Cornell Med Coll, New York, NY 10065 USA
来源：
MOLECULAR IMAGING AND BIOLOGY | 2015年 / 17卷 / 06期
基金：
美国国家科学基金会;
关键词：
PARP1; Glioblastoma; PET; Fluorescence; Multimodality; Imaging; U87; MG; IN-VIVO PET; OVARIAN-CANCER; SINGLE-CELL; ACID; INHIBITOR; RESECTION; OLAPARIB; DEFLUORINATION; EXCHANGE; THERAPY;
D O I：
10.1007/s11307-015-0858-0
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
The current study presents [F-18]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging. [F-18]PARPi-FL was obtained by F-19/F-18 isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model. [F-18]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [F-18]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 +/- 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography. The fluorescent component of [F-18]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [F-18]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.
引用
收藏
页码：848 / 855
页数：8
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