Evaluation of an adsorption-elution method for detection of astrovirus and norovirus in environmental waters

被引：70

作者：

Victoria, Matias ^{[1
]}

Guimaraes, Flavia ^{[1
]}

Fumian, Tulio ^{[1
]}

Ferreira, Fabiana ^{[1
]}

Vieira, Carmen ^{[1
]}

Leite, Jose Paulo ^{[1
]}

Miagostovich, Marize ^{[1
]}

机构：

[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Comparat Virol, BR-21040360 Rio De Janeiro, Brazil

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 156卷 / 1-2期

关键词：

Virus recovery; Astrovirus; Norovirus; Environmental water samples; HEPATITIS-A VIRUS; TIME RT-PCR; POLYMERASE-CHAIN-REACTION; RIO-DE-JANEIRO; ENTERIC VIRUSES; WASTE-WATER; ACUTE GASTROENTERITIS; MOLECULAR-DETECTION; ENTEROVIRUSES; JAPAN;

D O I：

10.1016/j.jviromet.2008.11.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human astroviruses (HAstV) and noroviruses (NoV) are shed frequently at high concentrations and persist for long periods in the environment, raising a significant health risk of water-related gastroenteritis. The aim of this study was to evaluate an adsorption-elution method with an HA (mixed cellulose esters) negatively charged membrane to determine the best recovery of HAstV and NoV from different environmental waters. As the presence Of MgCl2 affects viral adsorption onto the membrane, three different MgO2 concentrations were evaluated. The best recovery of both NoV and HAstV from mineral and river water samples was between 18% and 64%, while recovery from tap water and sea water samples was between 3% and 14%. These results suggest that detection and recovery of each enteric virus with this adsorption-elution method requires a specific MgCl2 concentration and depends on the source of environmental water tested. The combination of the HA negatively charged membrane to concentrate viruses with quantitative PCR detection allows for the identification of gastroenteritis viruses implicated in acute outbreaks of gastroenteritis. (c) 2008 Elsevier B.V. All rights reserved,

引用

页码：73 / 76

页数：4

共 33 条

[31] Prevalence and molecular epidemiology of noroviruses in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil, 2004 [J].

Victoria, Matias ;

Carvalho-Costa, Filipe Anibal ;

Heinemann, Marcos Bryan ;

Leite, Jose Paulo ;

Miagostovich, Marize .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2007, 26 (07) :602-606

[32] Molecular detection of hepatitis A virus in urban sewage in Rio de Janeiro, Brazil [J].

Villar, L. M. ;

de Paula, V. S. ;

Diniz-Mendes, L. ;

Guimaraes, F. R. ;

Ferreira, F. F. M. ;

Shubo, T. C. ;

Miagostovich, M. P. ;

Lampe, E. ;

Gaspar, A. M. C. .

LETTERS IN APPLIED MICROBIOLOGY, 2007, 45 (02) :168-173

[33] Evaluation of methods used to concentrate and detect hepatitis A virus in water samples [J].

Villar, Livia Melo ;

de Paula, Vanessa Salete ;

Diniz-Mendes, Leonardo ;

Lampe, Elisabeth ;

Coimbra Gaspar, Ana Maria .

JOURNAL OF VIROLOGICAL METHODS, 2006, 137 (02) :169-176

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