TiO2 Photocatalysis Damages Lipids and Proteins in Escherichia coli

被引:158
作者
Carre, Gaelle [1 ,2 ,3 ,4 ]
Hamon, Erwann [2 ,5 ]
Ennahar, Said [2 ,5 ]
Estner, Maxime [1 ,2 ]
Lett, Marie-Claire [4 ,6 ]
Horvatovich, Peter [7 ]
Gies, Jean-Pierre [1 ,2 ]
Keller, Valerie [3 ,4 ]
Keller, Nicolas [3 ,4 ]
Andre, Philippe [1 ,2 ]
机构
[1] CNRS, Lab Biophoton & Pharmacol, UMR 7213, Illkirch Graffenstaden, France
[2] Strasbourg Univ, Illkirch Graffenstaden, France
[3] CNRS, ICPEES, UMR 7515, Strasbourg, France
[4] Strasbourg Univ, Strasbourg, France
[5] CNRS, Lab Chim Analyt Mol BioAct, UMR 7178, Illkirch Graffenstaden, France
[6] CNRS, Lab Genet Mol, UMR 7156, Strasbourg, France
[7] Univ Groningen, Ctr Pharm, Dept Analyt Biochem, Groningen, Netherlands
关键词
HETEROGENEOUS PHOTOCATALYSIS; PROTEOMIC ANALYSIS; DISINFECTION; WATER; IDENTIFICATION; REMOVAL; CELLS; ACTIVATION; RESISTANCE; RADICALS;
D O I
10.1128/AEM.03995-13
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study investigates the mechanisms of UV-A (315 to 400 nm) photocatalysis with titanium dioxide (TiO2) applied to the degradation of Escherichia coli and their effects on two key cellular components: lipids and proteins. The impact of TiO2 photocatalysis on E. coli survival was monitored by counting on agar plate and by assessing lipid peroxidation and performing proteomic analysis. We observed through malondialdehyde quantification that lipid peroxidation occurred during the photocatalytic process, and the addition of superoxide dismutase, which acts as a scavenger of the superoxide anion radical (O-2 center dot(-)), inhibited this effect by half, showing us that O-2 center dot(-) radicals participate in the photocatalytic antimicrobial effect. Qualitative analysis using two-dimensional electrophoresis allowed selection of proteins for which spot modifications were observed during the applied treatments. Two-dimensional electrophoresis highlighted that among the selected protein spots, 7 and 19 spots had already disappeared in the dark in the presence of 0.1 g/liter and 0.4 g/liter TiO2, respectively, which is accounted for by the cytotoxic effect of TiO2. Exposure to 30 min of UV-A radiation in the presence of 0.1 g/liter and 0.4 g/liter TiO2 increased the numbers of missing spots to 14 and 22, respectively. The proteins affected by photocatalytic oxidation were strongly heterogeneous in terms of location and functional category. We identified several porins, proteins implicated in stress response, in transport, and in bacterial metabolism. This study reveals the simultaneous effects of O-2 center dot(-) on lipid peroxidation and on the proteome during photocatalytic treatment and therefore contributes to a better understanding of molecular mechanisms in antibacterial photocatalytic treatment.
引用
收藏
页码:2573 / 2581
页数:9
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