Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

被引:49
作者
Dehghani-Mohammadabadi, Maryam [1 ]
Salehi, Mohammad [2 ,3 ]
Farifteh, Fattaneh [2 ]
Nematollahi, Sedigheh [1 ]
Arefian, Ehsan [4 ]
Hajjarizadeh, Atena [4 ]
Parivar, Kazem [5 ]
Nourmohammadi, Zahra [5 ]
机构
[1] Stem Cell Technol Res Ctr, Dept Transgen Anim Sci, Tehran, Iran
[2] Shahid Beheshti Univ Med Sci, Cellular & Mol Biol Res Ctr, Tehran 193954717, Iran
[3] Shahid Beheshti Univ Med Sci, Fac Med, Dept Biotechnol, Tehran 193954717, Iran
[4] Stem Cell Technol Res Ctr, Dept Mol Biol & Genet Engn, Tehran, Iran
[5] Islamic Azad Univ, Sch Basic Sci, Sci & Res Branch, Dept Biol, Tehran, Iran
关键词
Melatonin; Vitrification; IVF; Reactive oxygen species; Apoptosis; IN-VITRO FERTILIZATION; MOUSE 2-CELL EMBRYOS; OXIDATIVE STRESS; EPITHELIAL-CELLS; IVM/IVF EMBRYOS; VITRIFICATION; BLASTOCYST; ANTIOXIDANT; APOPTOSIS; RECEPTOR;
D O I
10.1007/s10815-014-0172-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Antioxidant and anti-apoptotic effects of melatonin on development of in vitro fertilization (IVF)/vitrified two-cell mouse embryos were evaluated in this study. The IVF two-cell embryos were vitrified by cryotop, and were cultured in KSOM medium in different concentrations of melatonin (10(-6), 10(-9), 10(-12) M) and without melatonin. The blastocyst cell number, apoptotic cells and glutathione (GSH) level were evaluated by differential, TUNEL and cell tracker blue staining, respectively. The expression of Bax and Bcl-xl genes was evaluated by qPCR. The expression of melatonin receptors (Mtnr1a and Mtnr1b) in mouse 2-cell embryos and blastocysts was evaluated by RT-PCR. Melatonin increased the rate of cleavage and blastulation at 10(-12) M concentration (p < 0.05). The number of trophectoderm and inner cell mass showed a significant increase (p < 0.05) in 10(-9) M melatonin. The 10(-9) M and 10(-12) M melatonin treatments significantly reduced (p < 0.05) the apoptotic index. The significant increase in the expression of Bcl-xl observed at 10(-9) M concentration however, reduced expression of Bax was not statistically significant. The levels of GSH in 10(-9) and 10(-12) M groups were significantly improved relative to the control group (p < 0.05). The Mtnr1a was expressed in 2-cell embryos and blastocysts in all groups, but the expression of Mntr1b was not detected. Melatonin may have a special role against oxidative stress in protection of IVF/vitrified embryos.
引用
收藏
页码:453 / 461
页数:9
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