Inhibitory effects of three bisbenzylisoquinoline alkaloids on lipopolysaccharide-induced microglial activation

被引:23
|
作者
Meng, Xue-Lian [1 ]
Zheng, Liang-Chao [1 ]
Liu, Jia [1 ]
Gao, Cheng-Cheng [1 ]
Qiu, Ma-Chao [1 ]
Liu, Ying-Ying [1 ]
Lu, Jing [1 ]
Wang, Dan [2 ]
Chen, Chang-Lan [1 ]
机构
[1] Liaoning Univ, Sch Pharmaceut Sci, Shenyang 110036, Peoples R China
[2] Res Ctr Nat Prod Pharm Liaoning Prov, Shenyang 110036, Peoples R China
来源
RSC ADVANCES | 2017年 / 7卷 / 30期
基金
中国国家自然科学基金;
关键词
NF-KAPPA-B; NELUMBO-NUCIFERA; CELL APOPTOSIS; NITRIC-OXIDE; MUSCLE-CELLS; A549; CELLS; NEFERINE; MECHANISMS; LIENSININE; EMBRYO;
D O I
10.1039/c7ra01882g
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Activation of microglial cells has been considered to be associated with various neurodegenerative diseases. In this study, three bisbenzylisoquinoline alkaloids (liensinine, neferine, and isoliensinine) were found to inhibit microglial activation induced by lipopolysaccharide (LPS). Liensinine, neferine, and isoliensinine suppressed the release of NO and cytokines (TNF-alpha, IL-1 beta, and IL-6) by LPS-activated microglial cells. Three alkaloids displayed direct NO-scavenging activity in SNP solution and reduced LPS-induced protein and mRNA expression of iNOS in microglial cells. Further studies revealed that liensinine, neferine, and isoliensinine blocked I kappa B alpha phosphorylation and degradation, as well as ROS production in LPS-activated microglial cells. In addition, three alkaloids showed direct scavenging activity against DPPH, . OH, and ONOO radicals. These results suggest that liensinine, neferine, and isoliensinine may have potential in the treatment of neurodegenerative diseases accompanied by microglial activation.
引用
收藏
页码:18347 / 18357
页数:11
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