Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor

被引：40
作者：
Asakura, Ryo
Isobe, Tetsuhiko
Kurokawa, Kiyoshi
Aizawa, Hideki
Ohkubo, Michio
机构：
[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan
[2] Shiga Univ Med Sci, Dept Anat, Shiga 5202192, Japan
[3] Fukurawa Elect Co Ltd, Nishi Ku, Yokohama, Kanagawa 2200073, Japan
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 386卷 / 06期
关键词：
YAG:Ce3+; nanoparticle; 3-aminopropyltrimethoxysilane; avidin-biotin interaction; fluorescent image; biological application;
D O I：
10.1007/s00216-006-0814-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
YAG:Ce3+ stop nanoparticles 9.5 +/- 1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 degrees C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.
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收藏
页码：1641 / 1647
页数：7
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