Highly efficient in vitro adventitious shoot regeneration of peppermint (Mentha x piperita L.) using internodal explants

被引：5
作者：
Wang, Xiaohuan [2 ]
Gao, Zhenhua [2 ]
Wang, Yunzhen [2 ]
Bressan, Ray A. [3 ]
Weller, Stephen C. [3 ]
Li, Xia [1 ,2 ]
机构：
[1] Chinese Acad Sci, Ctr Agr Resources, Inst Genet & Dev Biol, Shijiazhuang 050021, Hebei, Peoples R China
[2] Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Ctr Agr Resources, Shijiazhuang 050021, Hebei, Peoples R China
[3] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2009年 / 45卷 / 04期
关键词：
Peppermint; Mentha x piperita; Internodes; Organogenesis; In vitro regeneration; SPEARMINT; TRANSFORMATION; MINT;
D O I：
10.1007/s11627-008-9170-x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
An in vitro regeneration system with a 100% efficiency rate was developed in peppermint [Mentha x piperita] using 5- to 7-mm-long second internode stem segments of 3-wk-old stock plants. Shoots developed at sites of excision on stem fragments either directly from the cells or via primary calluses. The optimal medium for maximum shoot initiation and regeneration contained Murashige and Skoog (MS) salts, B5 vitamins, thidiazuron (TDZ, 11.35 mu M), ZT (4.54 mu M), 10% coconut water (CW), 20 g l(-1) sucrose, 0.75% agar, adjusted to pH 5.8. A frequency of 100% shoot initiation was achieved, with an average of 39 shoots per explant. This regeneration system is highly reproducible. The regenerated plants developed normally and were phenotypically similar to Black Mitcham parents.
引用
收藏
页码：435 / 440
页数：6
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