Salinity and DOC Influence the Distribution of Free-living and Particle-attached Aerobic Anoxygenic Phototrophic Bacteria in the Qinghai-Tibetan Lakes

被引：4

作者：

Liu, Wen ^{[1
]}

Jiang, Hongchen ^{[1
]}

Yang, Jian ^{[1
]}

Wu, Geng ^{[1
]}

机构：

[1] China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan 430074, Hubei, Peoples R China

来源：

GEOMICROBIOLOGY JOURNAL | 2018年 / 35卷 / 03期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Aerobic anoxygenic phototrophic bacteria (AAPB); free-living; particle-attached; salinity; the Qinghai-Tibetan lakes; COMMUNITY STRUCTURE; DIVERSITY; ABUNDANCE; BACTERIOCHLOROPHYLL; WATERS; GRADIENT; ATLANTIC; DELAWARE; GENE; PUFM;

D O I：

10.1080/01490451.2017.1364805

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Aerobic anoxygenic phototrophic bacteria (AAPB) are widespread and play an important role in carbon cycling in the lakes of the Qinghai-Tibetan Plateau. However, little is known about how free-living and particle-attached AAPB distribute with salinity in the Qinghai-Tibetan lakes. In the present study, the abundance and diversity of free-living and particle-attached AAPB were investigated in seven Qinghai-Tibetan lakes with salinity ranging from freshwater to almost saturation (1.2 g L-1 241.1 g L-1). An integrated approach was employed including pufL-M gene (encoding the photosynthetic reaction center of AAPB) based quantitative polymerase chain reaction (qPCR) and PCR-cloning phylogenetic analysis. The qPCR data showed that the ratio between particle-attached and free-living AAPB was positively correlated (R-2 = 0.73, p < 0.01) with increasing salinity. The phylogenetic analysis of pufL-M gene showed that the composition of AAPB population varied with salinity in the studied Qinghai-Tibetan lakes. These results suggested that salinity may be the important factor shaping the AAPB distribution in the studied Qinghai-Tibetan lakes, and AAPB may be adapted to the harsh conditions in the Qinghai-Tibetan lakes by attaching to particles.

引用

页码：247 / 254

页数：8

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