Resistance Mechanisms and Molecular Docking Studies of Four Novel QoI Fungicides in Peronophythora litchii

被引：36

作者：

Zhou, Yuxin ^{[1
]}

Chen, Lei ^{[2
]}

Hu, Jian ^{[3
]}

Duan, Hongxia ^{[4
]}

Lin, Dong ^{[1
]}

Liu, Pengfei ^{[1
]}

Meng, Qingxiao ^{[1
]}

Li, Bin ^{[5
]}

Si, Naiguo ^{[5
]}

Liu, Changling ^{[5
]}

Liu, Xili ^{[1
]}

机构：

[1] China Agr Univ, Dept Plant Pathol, Coll Agr & Biotechnol, Beijing 100193, Peoples R China

[2] Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China

[3] Nanjing Agr Univ, Coll Agrograssland Sci, Nanjing 210095, Jiangsu, Peoples R China

[4] China Agr Univ, Dept Chem, Coll Sci, Beijing 100193, Peoples R China

[5] Shenyang Res Inst Chem Ind Co Ltd, State Key Lab Discovery & Dev Novel Pesticide, Shenyang 110021, Peoples R China

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

国家高技术研究发展计划(863计划);

关键词：

CYTOCHROME; INHIBITOR; FIELD; SITE; DNA;

D O I：

10.1038/srep17466

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Peronophythora litchii is the causal agent of litchi downy blight. Enestroburin, SYP-1620, SYP-2815 and ZJ0712 are four novel QoI fungicides developed by China. Eight mutants of P. litchii resistant to these QoI fungicides and azoxystrobin (as a known QoI fungicide) were obtained in our preliminary work. In this study, the full length of the cytochrome b gene in P. litchii, which has a full length of 382 amino acids, was cloned from both sensitive isolates and resistant mutants, and single-site mutations G142A, G142S, Y131C, or F128S were found in resistant mutants. Molecular docking was used to predict how the mutations alter the binding of the five QoI fungicides to the Qo-binding pockets. The results have increased our understanding of QoI fungicide-resistance mechanisms and may help in the development of more potent inhibitors against plant diseases in the fields.

引用

页数：10

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