Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor

Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not self-administer alcohol to levels necessary to achieve physiological and behavioral aspects of dependence. The protocol described herein can be utilized to induce alcohol dependence in rats by administering alcohol vapor to the homecage for an extended period of time. This method allows the researcher to control the level, duration, and pattern of intoxication, and it reliably induces physiological and behavioral aspects of alcohol dependence, allowing for the study of biology and behavior with relevance for AUD in humans.