Phase Coexistence in Single-Lipid Membranes Induced by Buffering Agents

被引：13

作者：

Johnson, Merrell A. ^{[1
]}

Seifert, Soenke ^{[2
]}

Petrache, Horia I. ^{[1
]}

Kimbe-Hill, Ann C. ^{[3
]}

机构：

[1] Indiana Univ Purdue Univ, Dept Phys, Indianapolis, IN 46202 USA

[2] Argonne Natl Lab, Adv Photon Source, Xray Sci Div, Argonne, IL 60439 USA

[3] Indiana Univ Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA

来源：

LANGMUIR | 2014年 / 30卷 / 33期

基金：

美国国家卫生研究院;

关键词：

BILAYERS; PHOSPHOLIPIDS; STABILIZATION; SEPARATION; REPULSION; PROTEINS;

D O I：

10.1021/la5018938

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recent literature has shown that buffers affect the interaction between lipid bilayers through a mechanism that involves van der Waals forces, electrostatics, hydration forces and membrane bending rigidity. This letter shows an additional peculiar effect of buffers on the mixed chain 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayers, namely phase coexistence similar to what was reported by Rappolt et al. for alkali chlorides. The data presented suggest that one phase appears to dehydrate below the value in pure water, while the other phase swells as the concentration of buffer is increased. However, since the two phases must be in osmotic equilibrium with one another, this behavior challenges theoretical models of lipid interactions.

引用

页码：9880 / 9885

页数：6

共 34 条

[1] Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity [J].

Ahola, T ;

Lampio, A ;

Auvinen, P ;

Kääriäinen, L .

EMBO JOURNAL, 1999, 18 (11) :3164-3172

[2] ALTERATION OF THE KINETIC PROPERTIES OF AN ENZYME BY THE BINDING OF BUFFER, INHIBITOR, OR SUBSTRATE [J].

ALBERTY, RA ;

BOCK, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1953, 39 (09) :895-900

[3] Compatibility of osmolytes with Gibbs energy of stabilization of proteins [J].

Anjum, F ;

Rishi, V ;

Ahmad, F .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1476 (01) :75-84

[4] THE STABILIZATION OF PROTEINS BY OSMOLYTES [J].

ARAKAWA, T ;

TIMASHEFF, SN .

BIOPHYSICAL JOURNAL, 1985, 47 (03) :411-414

[5]

Bancroft W. D., 1897, J PHYS CHEM

[6] Melittin-induced leakage from phosphatidylcholine vesicles is modulated by cholesterol: A property used for membrane targeting [J].

Benachir, T ;

Monette, M ;

Grenier, J ;

Lafleur, M .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1997, 25 (03) :201-210

[7] Entrapment of highly active membrane-bound receptors in macroporous sol-gel derived silica [J].

Besanger, TR ;

Easwaramoorthy, B ;

Brennan, JD .

ANALYTICAL CHEMISTRY, 2004, 76 (21) :6470-6475

[8] Buffers Affect the Bending Rigidity of Model Lipid Membranes [J].

Bouvrais, Helene ;

Duelund, Lars ;

Ipsen, John H. .

LANGMUIR, 2014, 30 (01) :13-16

[9] An Outer Membrane Protein Undergoes Enthalpy- and Entropy-Driven Transitions [J].

Cheneke, Belete R. ;

Indic, Mridhu ;

van den Berg, Bert ;

Movileanu, Liviu .

BIOCHEMISTRY, 2012, 51 (26) :5348-5358

[10] THERMAL-MECHANICAL FLUCTUATIONS ENHANCE REPULSION BETWEEN BIOMOLECULAR LAYERS [J].

EVANS, EA ;

PARSEGIAN, VA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) :7132-7136

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