One-step nucleic acid amplification-a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group

被引：93

作者：

Schem, Christian ^{[1
]}

Maass, Nicolai ^{[1
]}

Bauerschlag, Dirk O. ^{[1
]}

Carstensen, Martin H. ^{[4
]}

Loning, Thomas ^{[3
]}

Roder, Christian ^{[3
]}

Batic, Olivera ^{[2
]}

Jonat, Walter ^{[1
]}

Tiemann, Katharina ^{[2
]}

机构：

[1] Univ Clin Schleswig Holstein, Dept Obstet & Gynecol, D-24105 Kiel, Germany

[2] Univ Clin Schleswig Holstein, Dept Pathol, Kiel, Germany

[3] Albertinen Hosp, Dept Pathol, Hamburg, Germany

[4] Albertinen Hosp, Dept Gynecol & Obstet, Hamburg, Germany

来源：

VIRCHOWS ARCHIV | 2009年 / 454卷 / 02期

关键词：

OSNA; Breast cancer; Intraoperative diagnosis; CK19; Sentinel lymph node; MEDIATED ISOTHERMAL AMPLIFICATION; INTRAOPERATIVE DETECTION; BIOPSY; EXPRESSION; STANDARD;

D O I：

10.1007/s00428-008-0703-9

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Sentinel lymph node (SN) biopsy is part of the staging procedure in breast cancer patients. In this study, we compared an intraoperative tool named one-step nucleic acid amplification (OSNA) to our routine histological investigation. OSNA consists of a short homogenization step followed by amplification of cytokeratin (CK) 19 mRNA directly from the lysate. To evaluate the performance of OSNA in comparison to histology, analysis of 343 axillary lymph nodes (ALN) from 93 breast cancer patients was performed with both methods. Discordant samples were subjected to other methods. If these tests supported the OSNA results, these samples were excluded from the study. The concordance rate was 91.8%, sensitivity 98.1%, and specificity 90.8% before and 95.5%, 100%, and 95.6%, respectively, after discordant case investigation. Our results show that OSNA is an excellent method for the detection of metastases in lymph nodes and can be applied as an intraoperative diagnostic approach.

引用

页码：203 / 210

页数：8

共 30 条

[1] Sentinel node staging for breast cancer: intraoperative molecular pathology overcomes conventional histologic sampling errors [J].

Blumencranz, Peter ;

Whitworth, Pat W. ;

Deck, Kenneth ;

Rosenberg, Anne ;

Reintgen, Douglas ;

Beitsch, Peter ;

Chagpar, Anees ;

Julian, Thomas ;

Saha, Sukamal ;

Mamounas, Eleftherios ;

Giuliano, Armando ;

Simmons, Rache .

AMERICAN JOURNAL OF SURGERY, 2007, 194 (04) :426-432

[2] The results of frozen section, touch preparation, and cytological smear are comparable for intraoperative examination of sentinel lymph nodes: A study in 133 breast cancer patients [J].

Brogi, E ;

Torres-Matundan, E ;

Tan, LK ;

Cody, HS .

ANNALS OF SURGICAL ONCOLOGY, 2005, 12 (02) :173-180

[3] Keratin expression in human tissues and neoplasms [J].

Chu, PG ;

Weiss, LM .

HISTOPATHOLOGY, 2002, 40 (05) :403-439

[4] Intraoperative evaluation of sentinel lymph nodes for metastatic breast carcinoma by imprint cytology [J].

Creager, AJ ;

Geisinger, KR ;

Shiver, SA ;

Perrier, ND ;

Shen, P ;

Shaw, JA ;

Young, PR ;

Levine, EA .

MODERN PATHOLOGY, 2002, 15 (11) :1140-1147

[5] Pathological work-up of sentinel lymph nodes in breast cancer. Review of current data to be considered for the formulation of guidelines [J].

Cserni, G ;

Amendoeira, I ;

Apostolikas, N ;

Bellocq, JP ;

Bianchi, S ;

Bussolati, G ;

Boecker, W ;

Borisch, B ;

Connolly, CE ;

Decker, T ;

Dervan, P ;

Drijkoningen, M ;

Ellis, IO ;

Elston, CW ;

Eusebi, V ;

Faverly, D ;

Heikkila, P ;

Holland, R ;

Kerner, H ;

Kulka, J ;

Jacquemier, J ;

Lacerda, M ;

Martinez-Penuela, J ;

De Miguel, C ;

Peterse, JL ;

Rank, F ;

Regitnig, P ;

Reiner, A ;

Sapino, A ;

Sigal-Zafrani, B ;

Tanous, AM ;

Thorstenson, S ;

Zozaya, E ;

Wells, CA .

EUROPEAN JOURNAL OF CANCER, 2003, 39 (12) :1654-1667

[6] Discrepancies in current practice of pathological evaluation of sentinel lymph nodes in breast cancer. Results of a questionnaire based survey by the European Working Group for Breast Screening Pathology [J].

Cserni, G ;

Amendoeira, I ;

Apostolikas, N ;

Bellocq, JP ;

Bianchi, S ;

Boecker, W ;

Borisch, B ;

Connolly, CE ;

Decker, T ;

Dervan, P ;

Drijkoningen, M ;

Ellis, IO ;

Elston, CW ;

Eusebi, V ;

Faverly, D ;

Heikkila, P ;

Holland, R ;

Kerner, H ;

Kulka, J ;

Jacquemier, J ;

Lacerda, M ;

Martinez-Penuela, J ;

De Miguel, C ;

Peterse, JL ;

Rank, F ;

Regitnig, P ;

Reiner, A ;

Sapino, A ;

Sigal-Zafrani, B ;

Tanous, AM ;

Thorstenson, S ;

Zozaya, E ;

Fejes, G ;

Wells, CA .

JOURNAL OF CLINICAL PATHOLOGY, 2004, 57 (07) :695-701

[7]

DANIELE L, 2008, J CELL MOL IN PRESS

[8] Sentinel lymph node biopsy as the new standard of care in the surgical treatment for breast cancer [J].

Ferrari, Alberta ;

Rovera, Francesca ;

Dionigi, Paolo ;

Limonta, Giorgio ;

Marelli, Marina ;

Besana Ciani, Isabella ;

Bianchi, Veronica ;

Vanoli, Cristiana ;

Dionigi, Renzo .

EXPERT REVIEW OF ANTICANCER THERAPY, 2006, 6 (10) :1503-1515

[9] Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis -: An interim analysis of a prospective multi-institutional cohort study [J].

Gillanders, WE ;

Mikhitarian, K ;

Hebert, R ;

Mauldin, PD ;

Palesch, Y ;

Walters, C ;

Urist, MM ;

Mann, GB ;

Doherty, G ;

Herrmann, VM ;

Hill, AD ;

Eremin, O ;

El-Sheemy, M ;

Orr, RK ;

Valle, AA ;

Henderson, MA ;

Dewitty, RL ;

Sugg, SL ;

Frykberg, E ;

Yeh, K ;

Bell, RM ;

Metcalf, JS ;

Elliott, BM ;

Brothers, T ;

Robison, J ;

Mitas, M ;

Cole, DJ .

ANNALS OF SURGERY, 2004, 239 (06) :828-837

[10] Correlation between molecular metastases in sentinel lymph nodes of breast cancer patients and St Gallen risk category [J].

Gimbergues, P. ;

Dauplat, M. M. ;

Cayre, A. ;

Durando, X. ;

Le Bouedec, G. ;

Finat-Duclos, F. ;

Portefaix, G. ;

Kwiatkowski, F. ;

Dauplat, J. ;

Penault-Llorca, E. ;

Tchirkov, A. .

EJSO, 2007, 33 (01) :16-22

← 1 2 3 →