A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies

被引：57

作者：

Abdulrahman, Wassim ^{[1
]}

Uhring, Muriel ^{[1
]}

Kolb-Cheynel, Isabelle ^{[1
]}

Garnier, Jean-Marie ^{[1
]}

Moras, Dino ^{[1
]}

Rochel, Natacha ^{[1
]}

Busso, Didier ^{[1
]}

Potersman, Arnaud ^{[1
]}

机构：

[1] Inst Genet & Biol Mol & Cellulaire, UMR 7104, F-67404 Illkirch Graffenstaden, France

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 385卷 / 02期

关键词：

PROTEIN EXPRESSION; ESCHERICHIA-COLI; SYSTEM; THROUGHPUT; INSECT; CELLS;

D O I：

10.1016/j.ab.2008.10.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：383 / 385

页数：3

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