共 50 条
Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury
被引:50
|作者:
Kapur, Rick
[1
]
Kim, Michael
[2
,3
]
Rebetz, Johan
[1
,5
]
Hallstrom, Bjom
[4
]
Bjorkman, Jonas T.
Takabe-French, Alisa
[2
,3
]
Kim, Noel
[2
,3
]
Liu, Jonathan
[2
,3
]
Shanmugabhavananthan, Shanjeevan
[2
,3
]
Milosevic, Stefan
[2
,3
]
McVey, Mark J.
[2
,3
,6
,7
,8
,9
]
Speck, Edwin R.
[2
,3
]
Semple, John W.
[1
,2
,3
,6
,10
,11
,12
]
机构:
[1] Lund Univ, Div Hematol & Transfus Med, Lund, Sweden
[2] St Michaels Hosp, Keenan Res Ctr Biomed Sci, Toronto, ON, Canada
[3] St Michaels Hosp, Toronto Platelet Immunobiol Grp, Toronto, ON, Canada
[4] Lund Univ, Ctr Translat Genom, Lund, Sweden
[5] Skane Univ Hosp, Ctr Mol Diagnost, Lund, Sweden
[6] Canadian Blood Serv, Toronto, ON, Canada
[7] Univ Toronto, Dept Anesthesia, Toronto, ON, Canada
[8] Univ Toronto, Dept Physiol, Toronto, ON, Canada
[9] Hosp Sick Children, Dept Anesthesia & Pain Med, Toronto, ON, Canada
[10] Univ Toronto, Dept Pharmacol, Toronto, ON, Canada
[11] Univ Toronto, Dept Med, Toronto, ON, Canada
[12] Univ Toronto, Dept Lab Med & Pathol, Toronto, ON, Canada
基金:
瑞典研究理事会;
关键词:
GUT MICROBIOTA;
ULCERATIVE-COLITIS;
MOUSE MODEL;
DISEASE;
ANTIBODY;
FLORA;
HOMEOSTASIS;
RECEPTORS;
RISK;
MICE;
D O I:
10.1182/bloodadvances.2018018903
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.
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页码:1651 / 1663
页数:13
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