Advanced Artificial Extracellular Matrices Using Amphiphilic Nanogel-Cross-Linked Thin Films To Anchor Adhesion Proteins and Cytokines

被引：11

作者：

Hashimoto, Yoshihide ^{[1
,2
,3
]}

Mukai, Sada-atsu ^{[1
,2
,3
]}

Sawada, Shin-ichi ^{[1
,2
]}

Sasaki, Yoshihiro ^{[1
]}

Akiyoshi, Kazunari ^{[1
,2
]}

机构：

[1] Kyoto Univ, Grad Sch Engn, Dept Polymer Chem, Nishikyo Ku, Kyoto 6158510, Japan

[2] Japan Sci & Technol Agcy JST, Exploratory Res Adv Technol ERATO, Katsura Inttech Ctr, Nishikyo Ku, Kyoto 6158530, Japan

[3] Tokyo Med & Dent Univ, Dept Mat Based Med Engn, Inst Biomat & Bioengn, Chiyoda Ku, 2-3-10 Kanda Surugadai, Tokyo 1010062, Japan

来源：

ACS BIOMATERIALS SCIENCE & ENGINEERING | 2016年 / 2卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

self-assembled nanogels; nanogel tectonics; bioinspired artificial extracellular matrix; chaperone-like activity; tissue engineering; ASSEMBLED HYDROGEL NANOPARTICLE; BONE MORPHOGENETIC PROTEIN-2; FIBROBLAST-GROWTH-FACTOR; BIOMEDICAL APPLICATIONS; SYNTHETIC BIOMATERIALS; BEARING PULLULAN; FACTOR DELIVERY; PEG HYDROGELS; CELLS; HEPARIN;

D O I：

10.1021/acsbiomaterials.5b00485

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

A novel type of nanogel-cross-linked (NanoClik) film composed of acryloyl-modified cholesterol-bearing pullulan nanogels with pentaerythritol tetra(mercaptoethyl)polyoxyethylene as a cross-linker is created through the Michael addition coupled with solvent evaporation. Tensile testing and atomic force microscopy show that the elastic property of the NanoClik films can be controlled by changing the cross-linker concentration. The NanoClik films strongly absorb proteins after simple immersion in solutions of functional proteins, including the hormone insulin, cytokine bone morphogenetic protein-2 (BMP-2), and vitronectin. The amphiphilic nanogels in the films induce this absorption by acting as anchoring and loading proteins. Mouse embryo fibroblast cells adhere to and proliferate on the NanoClik films anchoring vitronectin, while NanoClik films loaded with BMP-2 strongly increase the differentiation of human mesenchymal stem cells into osteoblasts. These results suggest that the NanoClik films act as a novel artificial extracellular matrix that enables the reservation of various biological proteins to the nanogels.

引用

页码：375 / 384

页数：10

共 48 条

[1] Self-assembled hydrogel nanoparticle of cholesterol-bearing pullulan as a carrier of protein drugs: Complexation and stabilization of insulin [J].