Low-level laser irradiation at a high power intensity increased human endothelial cell exosome secretion via Wnt signaling

被引：57

作者：

Bagheri, Hesam Saghaei ^{[1
]}

Mousavi, Monireh ^{[2
]}

Rezabakhsh, Aysa ^{[1
]}

Rezaie, Jafar ^{[1
]}

Rasta, Seyed Hossein ^{[3
,4
]}

Nourazarian, Alireza ^{[5
,6
]}

Avci, Cigir Biray ^{[7
]}

Tajalli, Habib ^{[8
]}

Talebi, Mehdi ^{[9
]}

Oryan, Ahmad ^{[10
]}

Khaksar, Majid ^{[1
]}

Kazemi, Masoumeh ^{[1
]}

Nassiri, Seyed Mahdi ^{[11
]}

Ghaderi, Shahrooz ^{[12
]}

Bagca, Bakiye Goker ^{[7
]}

Rahbarghazi, Reza ^{[1
,13
]}

Sokullu, Emel ^{[14
,15
]}

机构：

[1] Tabriz Univ Med Sci, Stem Cell Res Ctr, Imam Reza St,Golgasht St, Tabriz 5166614756, Iran

[2] Islamic Azad Univ, Ahar Branch, Dept Genet, Ahar, Iran

[3] Tabriz Univ Med Sci, Fac Med, Dept Med Phys, Tabriz, Iran

[4] Univ Aberdeen, Sch Med Sci, Aberdeen, Scotland

[5] Tabriz Univ Med Sci, Dept Biochem, Fac Med, Tabriz, Iran

[6] Tabriz Univ Med Sci, Clin Labs, Fac Med, Tabriz, Iran

[7] Ege Univ, Dept Med Biol, Fac Med, Izmir, Turkey

[8] Univ Tabriz, Res Inst Appl Phys & Astron, Tabriz, Iran

[9] Tabriz Univ Med Sci, Hematol & Oncol Res Ctr, Tabriz, Iran

[10] Shiraz Univ, Dept Pathobiol, Fac Vet Med, Shiraz, Iran

[11] Univ Tehran, Dept Clin Pathol, Fac Vet Med, Tehran, Iran

[12] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Mol Med, Tabriz, Iran

[13] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Appl Cell Sci, Tabriz, Iran

[14] Izmir Katip Celebi Univ, Dept Bioengn, TR-35100 Izmir, Turkey

[15] Harvard Med Sch, Brigham & Womens Hosp, Div Biomed Engn, Harvard MIT Hlth Sci & Technol, Cambridge, MA 02139 USA

来源：

LASERS IN MEDICAL SCIENCE | 2018年 / 33卷 / 05期

关键词：

Human endothelial cells; Low-level laser irradiation; Exosome biogenesis; Angiogenesis; Wnt signaling pathway; MESENCHYMAL STEM-CELLS; APOPTOSIS; ANGIOGENESIS; RADIATION; GROWTH;

D O I：

10.1007/s10103-018-2495-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The distinct role of low-level laser irradiation (LLLI) on endothelial exosome biogenesis remains unclear. We hypothesize that laser irradiation of high dose in human endothelial cells (ECs) contributes to the modulation of exosome biogenesis via Wnt signaling pathway. When human ECs were treated with LLLI at a power density of 80 J/cm(2), the survival rate reduced. The potential of irradiated cells to release exosomes was increased significantly by expressing genes CD63, Alix, Rab27a, and b. This occurrence coincided with an enhanced acetylcholine esterase activity, pseudopodia formation, and reduced zeta potential value 24 h post-irradiation. Western blotting showed the induction of LC3 and reduced level of P62, confirming autophagy response. Flow cytometry and electron microscopy analyses revealed the health status of the mitochondrial function indicated by normal Delta I<spacing diaeresis> activity without any changes in the transcription level of PINK1 and Optineurin. When cells exposed to high power laser irradiation, p-Akt/Akt ratio and in vitro tubulogenesis capacity were blunted. PCR array and bioinformatics analyses showed the induction of transcription factors promoting Wnt signaling pathways and GTPase activity. Thus, LLLI at high power intensity increased exosome biogenesis by the induction of autophagy and Wnt signaling. LLLI at high power intensity increases exosome biogenesis by engaging the transcription factors related to Wnt signaling and autophagy stimulate.

引用

页码：1131 / 1145

页数：15

共 37 条

[1] Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability [J].