Preparation of Magnetic Molecularly Imprinted Polymers for Selective Recognition and Determination of Clenbuterol in Pork Samples

Magnetic molecularly imprinted polymer (MMIP) was successfully synthesized with acrylamide as a functional monomer and clenbuterol (CLB) as a template molecule. The synthesized MMIPs were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectrometry (FT-IR). MMIPs were used to identify and bind CLB as a solid phase extraction material. The experiment data were fitted by the Freundlich isotherm adsorption model. The results show that MMIPs have excellent recognition performance for CLB. MMIPs were successfully applied as adsorbents to preconcentrated CLB in pork samples and detected by HPLC with UV. The limit of detection (LOD) and limit of quantification (LOQ) were 4.27 mu g/L and 14.2 mu g/L, respectively. The spiked recovery rates ranged from 94.44% to 102.29%. Therefore, the prepared MMIPs can be used for selective preconcentration of CLB content in complex animal-derived food samples.