Effective antidiabetic and antioxidant fractions of Otostegia persica extract and their constituents

被引:18
作者
Tofighi, Zahra [1 ]
Alipour, Fatemeh [1 ]
Hadavinia, Hormoz [1 ]
Abdollahi, Mohammad [2 ]
Hadjiakhoondi, Abbas [1 ]
Yassa, Narguess [1 ]
机构
[1] Univ Tehran Med Sci, Med Plant Res Ctr, Fac Pharm, Dept Pharmacognosy, Tehran 1417414411, Iran
[2] Univ Tehran Med Sci, Pharmaceut Sci Res Ctr, Fac Pharm, Dept Pharmacol & Toxicol, Tehran 1417414411, Iran
关键词
Apigenin derivatives; chrysoeriol; hypoglycemic effect; radical scavenging activity; INDUCED DIABETIC-RATS; LIPID-PEROXIDATION; BURM; BOISS; STREPTOZOTOCIN; MICE; PLANTS; CELLS;
D O I
10.3109/13880209.2013.874463
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Context: Otostegia persica (Burm.) Boiss. (Lamiaceae), "Goldar" in Persian, is widely used in the folk medicine of south Iran for control of diabetes mellitus. Objective: In the present study, hypoglycemic and antioxidant effects of different fractions of the O. persica extract were investigated and constituents of effective fractions were elucidated. Materials and method: Different concentrations (100-400 mg/kg) of aqueous infusion (AI) of flowering aerial parts of the plant (traditional preparation) and all fractions of the O. persica extract (i.p. injection) were tested for antidiabetic activity in streptozocin-induced diabetic NMRI mice. Blood glucose level was measured at time 0 and intervals of 1, 2, 4, and 6 h later. Antioxidant activities of different fractions of the plant extract and pure compounds (0.1, 0.5, and 1 mg/ml) were determined with the DPPH method. Four compounds were isolated and identified from potent fractions. Results and discussion: Antidiabetic activity demonstrated that the effect of the methanol fraction at a dose of 300 mg/kg was equivalent with glibenclamide, and at a dose of 400 mg/kg was comparable with glibenclamide and insulin (p > 0.05). The EC50 of the methanol fraction was 307.12 mg. Methanol and ethyl acetate fractions showed antioxidant activities (both IC50 equal to 0.49 mg/ml), so these fractions were selected for the purification of compounds. Chrysoeriol from ethyl acetate and three apigenin derivatives (6-methylapigenin, apigenin-7-O-glucoside, and echinaticin) from the methanol fraction were isolated and identified (new for the species). Chrysoeriol exhibited potent antioxidant activity comparable with vitamin E and BHT (p > 0.05). Conclusion: The present study confirmed the folklore usage of O. persica for antidiabetic properties.
引用
收藏
页码:961 / 966
页数:6
相关论文
共 31 条
  • [1] Asadipour A, 2004, CLIN EXP PHARM PHYSL, V31, pA51
  • [2] Asghari G, 2006, RES PHARM SCI, V1, P53
  • [3] Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component
    Attele, AS
    Zhou, YP
    Xie, JT
    Wu, JA
    Zhang, L
    Dey, L
    Pugh, W
    Rue, PA
    Polonsky, KS
    Yuan, CS
    [J]. DIABETES, 2002, 51 (06) : 1851 - 1858
  • [4] Ayatollahi SA, 2009, DARU, V17, P290
  • [5] Ayatollahi SAM, 2007, J CHEM SOC PAKISTAN, V29, P61
  • [6] Ayatollahi SAM, 2010, AFR J BIOTECHNOL, V9, P3645
  • [7] CHAKRAVARTHY B K, 1980, Indian Journal of Pharmacology, V12, P123
  • [8] Chayarop K., 2011, Pharm. Soc. Asia, V38, P13
  • [9] Medicinal plants in the healing of dry socket in rats: Microbiological and microscopic analysis
    de Melo, EJM
    Raposo, MJ
    Neto, JAL
    Diniz, MFA
    Marcelino, CAC
    Sant'Ana, AEG
    [J]. PHYTOMEDICINE, 2002, 9 (02) : 109 - 116
  • [10] Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction
    Demirtas, Ibrahim
    Erenler, Ramazan
    Elmastas, Mahfuz
    Goktasoglu, Ahmet
    [J]. FOOD CHEMISTRY, 2013, 136 (01) : 34 - 40