Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33

被引：5
|
作者：
Senchenkova, Sof'ya N. [1 ]
Hou, Wenqi [2 ]
Naumenko, Olesya I. [1 ,4 ]
Geng, Peng [3 ]
Shashkov, Alexander S. [1 ]
Perepelov, Andrei V. [1 ]
Yang, Bin [2 ]
Knirel, Yuriy A. [1 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, Key Lab Mol Microbiol & Technol, Minist Educ, TEDA Inst Biol Sci & Biotechnol,TEDA, Tianjin, Peoples R China
[3] Tianjin Med Univ, Sch Basic Med Sci, Tianjin 300070, Peoples R China
[4] DI Mendeleev Univ Chem Technol Russia, Russian Acad Sci, Higher Chem Coll, Moscow, Russia
来源：
CARBOHYDRATE RESEARCH | 2018年 / 460卷
基金：
俄罗斯科学基金会;
关键词：
Escherichia coli; Lipopolysaccharide; O-antigen; O-specific polysaccharide; Bacterial polysaccharide structure; O-antigen gene cluster; Glycerol phosphate; ANTIGEN; CLUSTER; BIOSYNTHESIS;
D O I：
10.1016/j.carres.2018.02.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O33 followed by gel-permeation chromatography on Sephadex G-50. The polysaccharide was found to contain glycerol 2-phosphate (Gro-2-P), and the following structure of its tetrasaccharide repeat was established by sugar analysis, dephosphorylation, and 1D and 2D H-1 and C-13 NMR spectroscopy: [GRAPHICS] The O33-antigen gene cluster was analyzed and found to be essentially consistent with the O-polysaccharide structure. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码：47 / 50
页数：4
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