Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity

被引：21

作者：

Farrer, RA ^{[1
]}

Previte, MJR

Olson, CE

Peyser, LA

Fourkas, JT

So, PTC

机构：

[1] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

[2] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

来源：

OPTICS LETTERS | 1999年 / 24卷 / 24期

关键词：

D O I：

10.1364/OL.24.001832

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present. single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes. (C) 1999 Optical Society of America OCIS codes: 180.2520, 190.4180.

引用

页码：1832 / 1834

页数：3

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