Analysis of colonic autofluorescence spectra using multivariate curve resolution alternating least squares

被引：4

作者：

Liu, Lina ^{[1
]}

Yang, Changshun ^{[2
]}

Li, Weihua ^{[2
]}

Li, Buhong ^{[1
]}

Xie, Shusen ^{[1
]}

机构：

[1] Fujian Normal Univ, Fujian Prov Key Lab Photon Technol, Minist Educ, Key Lab OptoElect Sci & Technol Med, Fuzhou 350007, Peoples R China

[2] Fujian Prov Hosp, Dept Surg Oncol, Fuzhou 350001, Peoples R China

来源：

CHINESE OPTICS LETTERS | 2014年 / 12卷 / 05期

关键词：

EXCITATION-EMISSION MATRICES; TISSUE; FLUORESCENCE;

D O I：

10.3788/COL201412.051705

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Autofluorescence (AF) spectra of colonic normal and adenocarcinoma tissues are measured under excitation of 337 nm and analyzed by multivariate curve resolution alternating least squares (MCR-ALS) method using non-negativity constraint. Collagen, nicotinamide adenine dinucleotide hydrate (NADH) and elastin are identified as the main contributing biomedical components. Fisher's discrminant anlysis (FDA) on the concentration profiles of the principle components (PCs) shows acceptable sensitivity, specificty and accuracy for discrminanting the adenocarcinoma tissues from the normal tissues. MCR-ALS is a powerful tool for characterzing the spectra profiles of the main biochemical components in neoplasm transformation.

引用

页数：4

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