Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

被引：5

作者：

Gimenez-Lirola, Luis G. ^{[1
]}

Gerber, Priscilla F. ^{[1
,2
]}

Rowland, Raymond R. ^{[3
]}

Halbur, Patrick G. ^{[1
]}

Huang, Yao-Wei ^{[4
,5
,6
]}

Meng, Xiang-Jin ^{[4
]}

Opriessnig, Tanja ^{[1
,7
,8
]}

机构：

[1] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Coll Vet Med, Ames, IA 50011 USA

[2] Univ Fed Minas Gerais, Dept Vet Prevent Med, Lab Pesquisa Virol Anim, BR-31270901 Belo Horizonte, MG, Brazil

[3] Kansas State Univ, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA

[4] Virginia Polytech Inst & State Univ, Ctr Mol Med & Infect Dis, Dept Biomed Sci & Pathobiol, Coll Vet Med, Blacksburg, VA 24061 USA

[5] Zhejiang Univ, Inst Prevent Vet Med, Coll Anim Sci, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

[6] Zhejiang Univ, Key Lab Anim Virol, Coll Anim Sci, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

[7] Univ Edinburgh, Roslin Inst, Roslin EH25 9RG, Midlothian, Scotland

[8] Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin EH25 9RG, Midlothian, Scotland

来源：

RESEARCH IN VETERINARY SCIENCE | 2014年 / 96卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Torque teno sus virus; TTSuV; Porcine reproductive and respiratory syndrome virus; PRRSV; Fluorescent microbead-based immunoassay; 4-plex; Antibody detection; SYNDROME PRRS VIRUS; MONOCLONAL-ANTIBODIES; NUCLEOCAPSID PROTEIN; UNITED-STATES; AFFECTED PIGS; PREVALENCE; DISEASE; ELISA; IMMUNOASSAYS; EXPRESSION;

D O I：

10.1016/j.rvsc.2014.02.014

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0,20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：543 / 550

页数：8

共 38 条

[1] Exploratory study of Torque teno sus viruses in pulmonary inflammatory lesions in pigs
Aramouni, M.
Martinez, J.
Nieto, D.
Kekarainen, T.
Segales, J.
[J]. VETERINARY MICROBIOLOGY, 2013, 162 (2-4) : 338 - 344
[2] Age-related tissue distribution of swine Torque teno sus virus 1 and 2
Aramouni, M.
Segales, J.
Cortey, M.
Kekarainen, T.
[J]. VETERINARY MICROBIOLOGY, 2010, 146 (3-4) : 350 - 353
[3] An efficient system for high-level expression and easy purification of authentic recombinant proteins
Catanzariti, AM
Soboleva, TA
Jans, DA
Board, PG
Baker, RT
[J]. PROTEIN SCIENCE, 2004, 13 (05) : 1331 - 1339
[4] Cavanagh D, 1997, ARCH VIROL, V142, P629
[5] Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates
Dea, S
Gagnon, CA
Mardassi, H
Pirzadeh, B
Rogan, D
[J]. ARCHIVES OF VIROLOGY, 2000, 145 (04) : 659 - 688
[6] An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen
Denac, H
Moser, C
Tratschin, JD
Hofmann, MA
[J]. JOURNAL OF VIROLOGICAL METHODS, 1997, 65 (02) : 169 - 181
[7] Applications of Luminex® xMAP™ technology for rapid, high-throughput multiplexed nucleic acid detection
Dunbar, SA
[J]. CLINICA CHIMICA ACTA, 2006, 363 (1-2) : 71 - 82
[8] Faaberg K.S., 2012, Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses, P796
[9] A molecular clock dates the common ancestor of European-type porcine reproductive and respiratory syndrome virus at more than 10 years before the emergence of disease
Forsberg, R
Oleksiewicz, MB
Petersen, AMK
Hein, J
Botner, A
Storgaard, T
[J]. VIROLOGY, 2001, 289 (02) : 174 - 179
[10] Porcine Torque teno virus: Determination of viral genomic loads by genogroup-specific multiplex rt-PCR, detection of frequent multiple infections with genogroups 1 or 2, and establishment of viral full-length sequences
Gallei, Andreas
Pesch, Stefan
Esking, Wiebke Schulze
Keller, Christoph
Ohlinger, Volker Fred
[J]. VETERINARY MICROBIOLOGY, 2010, 143 (2-4) : 202 - 212

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