Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast

被引：33

作者：

Vecer, Jaroslav ^{[1
]}

Vesela, Petra ^{[2
]}

Malinsky, Jan ^{[2
]}

Herman, Petr ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Math & Phys, Prague 12116 2, Czech Republic

[2] Acad Sci Czech Republic, Inst Expt Med, Prague 14220 4, Czech Republic

来源：

FEBS LETTERS | 2014年 / 588卷 / 03期

关键词：

Membrane microdomain; Lipid order; Fluidity; Fluorescence anisotropy; Time-resolved fluorescence; TRANS-PARINARIC ACID; SACCHAROMYCES-CEREVISIAE; FLUORESCENT-PROBES; PHOSPHOLIPID MEMBRANE; MOLECULAR ORDER; LIPID-BILAYERS; DOMAINS; RAFTS; ANISOTROPY; ENDOCYTOSIS;

D O I：

10.1016/j.febslet.2013.11.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：443 / 449

页数：7

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