Characterization of a putative glutathione S-transferase of the parasitic nematode Trichinella spiralis

被引：46
|
作者：
Liu, Chun Ying [1 ]
Ren, Hua Na [1 ]
Song, Yan Yan [1 ]
Sun, Ge Ge [1 ]
Liu, Ruo Dan [1 ]
Jiang, Peng [1 ]
Long, Shao Rong [1 ]
Zhang, Xi [1 ]
Wang, Zhong Quan [1 ]
Cui, Jing [1 ]
机构：
[1] Zhengzhou Univ, Med Coll, Dept Parasitol, 40 Daxue Rd, Zhengzhou 450052, Henan, Peoples R China
来源：
EXPERIMENTAL PARASITOLOGY | 2018年 / 187卷
基金：
中国国家自然科学基金;
关键词：
Trichinella spiralis; Glutathione S-transferase; Biological characteristics; Invasion; ADCC; INTESTINAL EPITHELIAL-CELLS; REAL-TIME PCR; IN-VITRO; NUDIX HYDROLASE; PROTECTIVE IMMUNITY; BIOCHEMICAL-CHARACTERIZATION; MOLECULAR-IDENTIFICATION; ARTIFICIAL DIGESTION; PROTEOMIC ANALYSIS; HUMAN TRICHINOSIS;
D O I：
10.1016/j.exppara.2018.02.005
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The aim of this study was to identify the biological characteristics and functions of a putative Trichinella spiralis glutathione S-transferase (TspGST). The results of real-time PCR and immunofluorescent test (IFT) showed that the TspGST gene was expressed at all of T. spiralis different developmental stages (muscle larvae, intestinal infective larvae, adult worms and newborn larvae). When anti-rTspGST serum, mouse infection serum, and pre-immune serum were added to the medium, the inhibition rate of the larvae penetrated into the intestinal epithelial cells (IECs) was 25.72%, 49.55%, and 4.51%, respectively (P < 0.01). The inhibition of anti-rTspGST serum on larval invasion of IECs was dose-dependent (P < 0.05). Anti-rTspGST antibodies killed T. spiralis newborn larvae by an ADCC-mediated mechanism. Our results showed that the TspGST seemed to be an indispensable protein for T spiralis invasion, growth and survival in host. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：59 / 66
页数：8
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